A second tumor necrosis factor receptor gene product can shed a naturally occurring tumor necrosis factor inhibitor

Tadahiko Kohno, Michael T. Brewer, Susan L. Baker, Phillip E. Schwartz, Michael King, Karin K. Hale, Charles H. Squires, Robert C. Thompson, James L. Vannice

Research output: Contribution to journalArticle

252 Citations (Scopus)

Abstract

An inhibitor of tumor necrosis factor (TNF) has been isolated from the human histiocytic lymphoma cell line U-937 that is capable of inhibiting both TNF-α and TNF-β. Protein sequencing has verified that it is distinct from a previously described TNF inhibitor that is a soluble fragment of a TNF receptor molecule (TNFrI). The cDNA sequence of this second TNF inhibitor clone suggests that it is also a soluble fragment of a TNF receptor. Expression of this cDNA sequence in COS-7 cells verified that it encodes a receptor for TNF-α (TNFrII) that can give rise to a soluble inhibitor of TNF-α, presumably through proteolytic cleavage. The extracellular domain of TNFrII has significant homology with that of TNFrI and these two receptors share a striking conservation of cysteine residue alignment with the extracellular domain of the nerve growth factor receptor. These three receptor molecules are therefore members of a family of polypeptide hormone receptors.

Original languageEnglish
Pages (from-to)8331-8335
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume87
Issue number21
StatePublished - 1990
Externally publishedYes

Fingerprint

Tumor Necrosis Factor Receptors
Tumor Necrosis Factor-alpha
Genes
Complementary DNA
Nerve Growth Factor Receptor
Lymphoma, Large B-Cell, Diffuse
Peptide Hormones
COS Cells
Protein Sequence Analysis
Cysteine
Clone Cells
Cell Line

Keywords

  • cDNA cloning
  • Gene expression
  • Lymphotoxin
  • Phorbol 12-myristate 13-acetate
  • Receptor shedding

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

A second tumor necrosis factor receptor gene product can shed a naturally occurring tumor necrosis factor inhibitor. / Kohno, Tadahiko; Brewer, Michael T.; Baker, Susan L.; Schwartz, Phillip E.; King, Michael; Hale, Karin K.; Squires, Charles H.; Thompson, Robert C.; Vannice, James L.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, No. 21, 1990, p. 8331-8335.

Research output: Contribution to journalArticle

Kohno, T, Brewer, MT, Baker, SL, Schwartz, PE, King, M, Hale, KK, Squires, CH, Thompson, RC & Vannice, JL 1990, 'A second tumor necrosis factor receptor gene product can shed a naturally occurring tumor necrosis factor inhibitor', Proceedings of the National Academy of Sciences of the United States of America, vol. 87, no. 21, pp. 8331-8335.
Kohno, Tadahiko ; Brewer, Michael T. ; Baker, Susan L. ; Schwartz, Phillip E. ; King, Michael ; Hale, Karin K. ; Squires, Charles H. ; Thompson, Robert C. ; Vannice, James L. / A second tumor necrosis factor receptor gene product can shed a naturally occurring tumor necrosis factor inhibitor. In: Proceedings of the National Academy of Sciences of the United States of America. 1990 ; Vol. 87, No. 21. pp. 8331-8335.
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