A selective screen reveals discrete functional domains in Drosophila Nanos

Gustavo Arrizabalaga, Ruth Lehmann

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The Drosophila protein Nanos encodes an evolutionarily conserved protein with two zinc finger motifs. in the embryo, Nanos protein function is required for establishment of the anterior-posterior body pattern and for the migration of primordial germ cells. During oogenesis, Nanos protein is involved in the establishment and maintenance of germ-line stem cells and the differentiation of oocyte precursor cells. To establish proper embryonic patterning, Nanos acts as a translational regulator of hunchback RNA. Nanos' targets for germ cell migration and development are not known. Here, we describe a selective genetic screen aimed at isolating new nanos alleles. The molecular and genetic analysis of 68 new alleles has allowed us to identify amino acids critical for nanos function. This analysis shows that the CCHC motifs, which coordinate two metal ions, are essential for all known functions of Nanos protein. Furthermore, a region C-terminal to the zinc fingers seems to constitute a novel functional domain within the Nanos protein. This 'tail region' of Nanos is required for abdomen formation and germ cell migration, but not for oogenesis.

Original languageEnglish (US)
Pages (from-to)1825-1838
Number of pages14
JournalGenetics
Volume153
Issue number4
StatePublished - Dec 1 1999

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Drosophila
Germ Cells
Oogenesis
Zinc Fingers
Proteins
Cell Movement
Alleles
Abdomen
Oocytes
Tail
Molecular Biology
Cell Differentiation
Stem Cells
Embryonic Structures
Metals
Maintenance
RNA
Ions
Amino Acids

ASJC Scopus subject areas

  • Genetics

Cite this

A selective screen reveals discrete functional domains in Drosophila Nanos. / Arrizabalaga, Gustavo; Lehmann, Ruth.

In: Genetics, Vol. 153, No. 4, 01.12.1999, p. 1825-1838.

Research output: Contribution to journalArticle

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