A sensitive and specific assay for plasminogen activators

Daniel Smith, James Harmon, Whyte G. Owen

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A simple, sensitive and specific assay for plasminogen activators is described. The assay utilizes fluorescein-labeled fibrinogen or fibrin at low concentrations, and enables simultaneous evaluation of the plasminogen and fibrin dependence of the reaction, that is, discrimination of tissue-type and urokinase-type plasminogen activators, and non-specific proteolysis. Addition of antisera verify identification of the activator species. The assay reagent contains plasminogen and fluorescein-labeled fibrinogen, to which is added the specimen and then then thrombin, either at the initiation or the termination of the reaction. Supernatant fluorescence is proportional to plasminogen activator concentration. With a four-hour incubation, 1 milliunit (14 pg) of tissue (melanoma) plasminogen activator (TPA) or 2 milliunit (36 pg) of urokinase (UK) may be detected.

Original languageEnglish (US)
Pages (from-to)533-541
Number of pages9
JournalThrombosis Research
Volume37
Issue number4
DOIs
StatePublished - Feb 15 1985
Externally publishedYes

Fingerprint

Plasminogen Activators
Plasminogen
Urokinase-Type Plasminogen Activator
Tissue Plasminogen Activator
Fibrin
Fluorescein
Fibrinogen
Thrombin
Proteolysis
Immune Sera
Melanoma
Fluorescence
2-(2-thienyl)propionic acid

Keywords

  • fibrinolysis
  • plasminogen activators

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Hematology

Cite this

A sensitive and specific assay for plasminogen activators. / Smith, Daniel; Harmon, James; Owen, Whyte G.

In: Thrombosis Research, Vol. 37, No. 4, 15.02.1985, p. 533-541.

Research output: Contribution to journalArticle

Smith, Daniel ; Harmon, James ; Owen, Whyte G. / A sensitive and specific assay for plasminogen activators. In: Thrombosis Research. 1985 ; Vol. 37, No. 4. pp. 533-541.
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