A simple and rapid method for the estimation of l-asparaginase in chromatographic and electrophoretic effluents: Comparison with other methods

Hiremagalur N. Jayaram, David A. Cooney, Shantha Jayaram, Larry Rosenblum

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

Four methods for the measurement of l-asparaginase have been compared with a new modification of an enzymatic, spectrophotometric technique utilizing l-glutamate oxaloacetate transaminase and malate dehydrogenase. This last technique uses disposable polycarbonate tubes both as reaction vessels and as cuvettes. Only a single addition of reagents and a single reading of absorbance is required. The simplicity of this technique is especially helpful when the prompt analysis of many samples of l-asparaginase is desired.

Original languageEnglish (US)
Pages (from-to)327-346
Number of pages20
JournalAnalytical biochemistry
Volume59
Issue number2
DOIs
StatePublished - Jun 1974

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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