A simple and rapid method for the estimation of l-asparaginase in chromatographic and electrophoretic effluents: Comparison with other methods

Hiremagalur N. Jayaram, David A. Cooney, Shantha Jayaram, Larry Rosenblum

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Four methods for the measurement of l-asparaginase have been compared with a new modification of an enzymatic, spectrophotometric technique utilizing l-glutamate oxaloacetate transaminase and malate dehydrogenase. This last technique uses disposable polycarbonate tubes both as reaction vessels and as cuvettes. Only a single addition of reagents and a single reading of absorbance is required. The simplicity of this technique is especially helpful when the prompt analysis of many samples of l-asparaginase is desired.

Original languageEnglish (US)
Pages (from-to)327-346
Number of pages20
JournalAnalytical Biochemistry
Volume59
Issue number2
DOIs
StatePublished - 1974
Externally publishedYes

Fingerprint

Asparaginase
polycarbonate
Effluents
Oxaloacetic Acid
Malate Dehydrogenase
Transaminases
Reading
Glutamic Acid

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

A simple and rapid method for the estimation of l-asparaginase in chromatographic and electrophoretic effluents : Comparison with other methods. / Jayaram, Hiremagalur N.; Cooney, David A.; Jayaram, Shantha; Rosenblum, Larry.

In: Analytical Biochemistry, Vol. 59, No. 2, 1974, p. 327-346.

Research output: Contribution to journalArticle

Jayaram, Hiremagalur N. ; Cooney, David A. ; Jayaram, Shantha ; Rosenblum, Larry. / A simple and rapid method for the estimation of l-asparaginase in chromatographic and electrophoretic effluents : Comparison with other methods. In: Analytical Biochemistry. 1974 ; Vol. 59, No. 2. pp. 327-346.
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