A simple automated method for continuous fieldwise measurement of microvascular hemodynamics

Sherry G. Clendenon, Xiao Fu, Robert A. Von Hoene, Jeffrey L. Clendenon, James P. Sluka, Seth Winfree, Henry Mang, Michelle Martinez, Adele J. Filson, James E. Klaunig, James A. Glazier, Kenneth Dunn

Research output: Contribution to journalArticle

Abstract

Microvascular perfusion dynamics are vital to physiological function and are frequently dysregulated in injury and disease. Typically studies measure microvascular flow in a few selected vascular segments over limited time, failing to capture spatial and temporal variability. To quantify microvascular flow in a more complete and unbiased way we developed STAFF (Spatial Temporal Analysis of Fieldwise Flow), a macro for FIJI open-source image analysis software. Using high-speed microvascular flow movies, STAFF generates kymographs for every time interval for every vascular segment, calculates flow velocities from red blood cell shadow angles, and outputs the data as color-coded velocity map movies and spreadsheets. In untreated mice, analyses demonstrated profound variation even between adjacent sinusoids over seconds. In acetaminophen-treated mice we detected flow reduction localized to pericentral regions. STAFF is a powerful new tool capable of providing novel insights by enabling measurement of the complex spatiotemporal dynamics of microvascular flow.

Original languageEnglish (US)
Pages (from-to)7-13
Number of pages7
JournalMicrovascular Research
Volume123
DOIs
StatePublished - May 1 2019

Fingerprint

Spatio-Temporal Analysis
Hemodynamics
Motion Pictures
Blood Vessels
Spreadsheets
Acetaminophen
Flow velocity
Image analysis
Macros
Blood
Cells
Color
Software
Perfusion
Erythrocytes
Wounds and Injuries

Keywords

  • Capillary
  • Hemodynamics
  • Intravital microscopy
  • Microvascular
  • Red blood cell velocity

ASJC Scopus subject areas

  • Biochemistry
  • Cardiology and Cardiovascular Medicine
  • Cell Biology

Cite this

A simple automated method for continuous fieldwise measurement of microvascular hemodynamics. / Clendenon, Sherry G.; Fu, Xiao; Von Hoene, Robert A.; Clendenon, Jeffrey L.; Sluka, James P.; Winfree, Seth; Mang, Henry; Martinez, Michelle; Filson, Adele J.; Klaunig, James E.; Glazier, James A.; Dunn, Kenneth.

In: Microvascular Research, Vol. 123, 01.05.2019, p. 7-13.

Research output: Contribution to journalArticle

Clendenon, SG, Fu, X, Von Hoene, RA, Clendenon, JL, Sluka, JP, Winfree, S, Mang, H, Martinez, M, Filson, AJ, Klaunig, JE, Glazier, JA & Dunn, K 2019, 'A simple automated method for continuous fieldwise measurement of microvascular hemodynamics', Microvascular Research, vol. 123, pp. 7-13. https://doi.org/10.1016/j.mvr.2018.11.010
Clendenon, Sherry G. ; Fu, Xiao ; Von Hoene, Robert A. ; Clendenon, Jeffrey L. ; Sluka, James P. ; Winfree, Seth ; Mang, Henry ; Martinez, Michelle ; Filson, Adele J. ; Klaunig, James E. ; Glazier, James A. ; Dunn, Kenneth. / A simple automated method for continuous fieldwise measurement of microvascular hemodynamics. In: Microvascular Research. 2019 ; Vol. 123. pp. 7-13.
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