A simplified method for generating oligodendrocyte progenitor cells from neural precursor cells isolated from the E16 rat spinal cord

Sai Li Fu, Jian Guo Hu, Ying Li, Yan Xia Wang, Jian Qiang Jin, Xiao Ming Xu, Pei Hua Lu

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Conditioned medium obtained from B104 neuroblastoma cells (B104CM) has been used widely for inducing oligodendrocyte progenitor cells (OPCs) from neural precursor cells (NPCs). Our previous studies have demonstrated that E16 rat spinal cord-derived NPCs could be induced to differentiate into OPCs using a combination of B104CM and basic fibroblast growth factor (bFGF). Here we report the development of a more efficient and reliable approach to generate large quantities of highly purified OPCs from spinal cord-derived NPCs using a combination of platelet derived growth factor (PDGF) and bFGF. We demonstrated that, after the two factors application, over 90% cells displayed typical bipolar or tripolar morphology and expressed markers for OPCs including A2B5 (90.36 ± 4.59%), NG2 (93.63 ± 3.37%) and platelet derived growth factor alpha receptor (PDGFR; 90.35 ± 1.95%). Our results indicated that the PDGF/bFGF combination is more efficient in generating OPCs than the B104CM/bFGF. And it is a more potent combination of factors in promoting proliferation of OPCs.

Original languageEnglish (US)
Pages (from-to)367-377
Number of pages11
JournalActa Neurobiologiae Experimentalis
Volume67
Issue number4
StatePublished - Dec 1 2007
Externally publishedYes

Keywords

  • B104CM
  • Cell culture
  • Differentiation
  • Neural precursor cells
  • Oligodendrocyte progenitor cell
  • PDGF
  • bFGF

ASJC Scopus subject areas

  • Neuroscience(all)

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