A specific RNA-protein interaction at yeast polyadenylation efficiency elements

Shaoxiong Chen, Linda E. Hyman

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The specific RNA-protein interactions responsible for the production of mature 3' ends of eukaryotic mRNAs are not well understood. Sequence elements at the 3' ends of yeast genes have been identified that specify the position of the poly(A) site and the efficiency of polyadenylation. To provide additional insights into the interaction between important sequences that direct 3'-end formation in vivo and nuclear proteins, we utilized gel mobility shift assays and UV-crosslinking studies. The data indicate that a protein, with an apparent molecular weight of 80 kDa, interacts specifically with pre-mRNA at the (UA)3 efficiency element. Although the interaction is specific, it can be competed by RNA sequences that do not contain the same type of efficiency element; that is, a sequence lacking a (UA)3 repeat. This result implies that the protein binding site is flexible. Using immunoprecipitation techniques, the protein has been identified as Hrp1, a heteronuclear RNA binding protein. The role of Hrp1p in 3'-end formation including RNA processing and transcription termination is addressed.

Original languageEnglish (US)
Pages (from-to)4965-4974
Number of pages10
JournalNucleic Acids Research
Volume26
Issue number21
DOIs
StatePublished - Nov 1 1998
Externally publishedYes

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Polyadenylation
Yeasts
RNA
Proteins
Poly A
RNA-Binding Proteins
RNA Precursors
Electrophoretic Mobility Shift Assay
Nuclear Proteins
Immunoprecipitation
Protein Binding
Molecular Weight
Gels
Binding Sites
Messenger RNA
Genes

ASJC Scopus subject areas

  • Genetics

Cite this

A specific RNA-protein interaction at yeast polyadenylation efficiency elements. / Chen, Shaoxiong; Hyman, Linda E.

In: Nucleic Acids Research, Vol. 26, No. 21, 01.11.1998, p. 4965-4974.

Research output: Contribution to journalArticle

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