A spoonful of sugar helps the medicine go down

A novel technique to improve oral gavage in mice

Amber F. Hoggatt, Jonathan Hoggatt, Meghan Honerlaw, Louis Pelus

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Oral gavage is a common route of precise oral dosing for studies in rodents. Complications including tracheal administration, esophageal trauma, and aspiration are common and usually related to animal resistance to the procedure, and the stress induced by oral gavage can be a confounding variable in many studies. The taste of sucrose conveys a pacifying and analgesic effect in newborns, whereas sour solutions can induce the swallow reflex in humans that are dysphagic. We hypothesized that precoating a gavage needle with sucrose or citrate (or both) would pacify mice and induce them to swallow, reducing the stress and complications associated with the technique. To validate this hypothesis, we quantitated time to passage, stress-related behavioral reactions to the procedure, and plasma corticosterone levels in mice after precoating gavage needles with water, sucrose, citrate, sucrose and citrate, or sodium chloride prior to oral gavage. Precoating needles with sucrose reduced the time to passage, decreased observable stress-related reactions to the procedure, and maintained plasma corticosterone levels similar to those in ungavaged control mice. Coating needles with water, sucrose and citrate, or citrate had no beneficial effects on these parameters. Our findings describe a novel, validated technique that measurably decreases signs of stress and thereby improves animal welfare during oral gavage. Furthermore, the use of sucrose may be a valuable tool to refine other minor or nonsurgical procedures in the field of laboratory animal research.

Original languageEnglish
Pages (from-to)329-334
Number of pages6
JournalJournal of the American Association for Laboratory Animal Science
Volume49
Issue number3
StatePublished - May 2010

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mouth
medicine
sucrose
citrates
sugars
mice
corticosterone
methodology
animal research
analgesic effect
reflexes
sodium chloride
animal welfare
coatings
laboratory animals
neonates
rodents
water
animals

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

A spoonful of sugar helps the medicine go down : A novel technique to improve oral gavage in mice. / Hoggatt, Amber F.; Hoggatt, Jonathan; Honerlaw, Meghan; Pelus, Louis.

In: Journal of the American Association for Laboratory Animal Science, Vol. 49, No. 3, 05.2010, p. 329-334.

Research output: Contribution to journalArticle

Hoggatt, Amber F. ; Hoggatt, Jonathan ; Honerlaw, Meghan ; Pelus, Louis. / A spoonful of sugar helps the medicine go down : A novel technique to improve oral gavage in mice. In: Journal of the American Association for Laboratory Animal Science. 2010 ; Vol. 49, No. 3. pp. 329-334.
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