A Study on the lack of [methyl-3H] thymidine uptake and incorporation by chemolithotrophic bacteria

Brian H. Johnstone, Ronald D. Jones

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Five chemolithotrophic bacteria were tested for their ability to incorporate [methyl-3H] thymidine. None of the bacteria incorporated the label, even after incubation for 24 hours. The inability of these bacteria to incorporate thymidine appears to be due to an absence of transport mechanisms for exogenous nucleosides. As a result of these findings, it is concluded that activities deduced from labeled thymidine incorporation measurements probably do not include the activity of chemolithotrophic bacteria.

Original languageEnglish (US)
Pages (from-to)73-77
Number of pages5
JournalMicrobial Ecology
Volume18
Issue number1
DOIs
StatePublished - Jul 1989
Externally publishedYes

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thymidine
Thymidine
Bacteria
uptake mechanisms
bacterium
bacteria
nucleosides
Nucleosides
incubation
incorporation

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology
  • Ecology
  • Aquatic Science

Cite this

A Study on the lack of [methyl-3H] thymidine uptake and incorporation by chemolithotrophic bacteria. / Johnstone, Brian H.; Jones, Ronald D.

In: Microbial Ecology, Vol. 18, No. 1, 07.1989, p. 73-77.

Research output: Contribution to journalArticle

Johnstone, Brian H. ; Jones, Ronald D. / A Study on the lack of [methyl-3H] thymidine uptake and incorporation by chemolithotrophic bacteria. In: Microbial Ecology. 1989 ; Vol. 18, No. 1. pp. 73-77.
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