A subpopulation of human polymorphonuclear neutrophils contains an active form of lactoferrin capable of binding to human monocytes and inhibiting production of granulocyte-macrophage colony stimulatory activities

H. E. Broxmeyer, P. Ralph, J. Bognacki, P. W. Kincade, M. Desousa

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

Subpopulations of human peripheral blood polymorphonuclear neutrophils (PMN) differing in functional capacity were separated by a rosetting procedure by using rabbit IgG antibody-coated sheep erythrocytes (EA). EA + and EA - populations of PMN both contained lactoferrin (LF) as determined by radioimmunoassay. However, only LF (10 -16 to 10 -6 M) obtained from EA + (= FC receptor (FcR) +) PMN was able to suppress the production of granulocyte-macrophage colony stimulatory activity derived from monocytes. LF from EA - PMN was inactive at concentrations as high as 10 -5 M. Extracts of EA - PMN contained proteolytic enzymes, not apparent in FcR + PMN, that inactivated the inhibitory activity of LF obtained from FcR + PMN. These results were substantiated by immunofluorescence studies. LF from FcR + PMN bound to monocytes whereas LF from EA - PMN demonstrated negligible binding to the monocytes and extracts from EA - PMN drastically decreased the capability of LF obtained from FcR + obtained from FcR + PMN to bind to monocytes. These studies demonstrate functional heterogeneity of peripheral blood PMN and suggest a role of PMN subpopulations in the regulation of myelopoiesis.

Original languageEnglish (US)
Pages (from-to)903-909
Number of pages7
JournalJournal of Immunology
Volume125
Issue number2
StatePublished - Nov 10 1980
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'A subpopulation of human polymorphonuclear neutrophils contains an active form of lactoferrin capable of binding to human monocytes and inhibiting production of granulocyte-macrophage colony stimulatory activities'. Together they form a unique fingerprint.

  • Cite this