A subset of toll-like receptor ligands induces cross-presentation by bone marrow-derived dendritic cells

Sandip K. Datta, Vanessa Redecke, Kiley R. Prilliman, Kenji Takabayashi, Maripat Corr, Thomas Tallant, Joseph DiDonato, Roman Dziarski, Shizuo Akira, Stephen P. Schoenberger, Eyal Raz

Research output: Contribution to journalArticle

237 Scopus citations

Abstract

Dendritic cells (DCs) are capable of cross-presenting exogenous Ag to CD8+ CTLs. Detection of microbial products by Toll-like receptors (TLRs) leads to activation of DCs and subsequent orchestration of an adaptive immune response. We hypothesized that microbial TLR ligands could activate DCs to cross-present Ag to CTLs. Using DCs and CTLs in an in vitro cross- presentation system, we show that a subset of microbial TLR ligands, namely ligands of TLR3 (poly(inosinic-cytidylic) acid) and TLR9 (immunostimulatory CpG DNA), induces cross-presentation. In contrast to presentation of Ag to CD4+ T cells by immature DCs, TLR-induced cross-presentation is mediated by mature DCs, is independent of endosomal acidification, and relies on cytosolic Ag processing machinery.

Original languageEnglish (US)
Pages (from-to)4102-4110
Number of pages9
JournalJournal of Immunology
Volume170
Issue number8
DOIs
StatePublished - Apr 15 2003

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Datta, S. K., Redecke, V., Prilliman, K. R., Takabayashi, K., Corr, M., Tallant, T., DiDonato, J., Dziarski, R., Akira, S., Schoenberger, S. P., & Raz, E. (2003). A subset of toll-like receptor ligands induces cross-presentation by bone marrow-derived dendritic cells. Journal of Immunology, 170(8), 4102-4110. https://doi.org/10.4049/jimmunol.170.8.4102