A subset of toll-like receptor ligands induces cross-presentation by bone marrow-derived dendritic cells

Sandip K. Datta, Vanessa Redecke, Kiley R. Prilliman, Kenji Takabayashi, Maripat Corr, Thomas Tallant, Joseph DiDonato, Roman Dziarski, Shizuo Akira, Stephen P. Schoenberger, Eyal Raz

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Abstract

Dendritic cells (DCs) are capable of cross-presenting exogenous Ag to CD8+ CTLs. Detection of microbial products by Toll-like receptors (TLRs) leads to activation of DCs and subsequent orchestration of an adaptive immune response. We hypothesized that microbial TLR ligands could activate DCs to cross-present Ag to CTLs. Using DCs and CTLs in an in vitro cross- presentation system, we show that a subset of microbial TLR ligands, namely ligands of TLR3 (poly(inosinic-cytidylic) acid) and TLR9 (immunostimulatory CpG DNA), induces cross-presentation. In contrast to presentation of Ag to CD4+ T cells by immature DCs, TLR-induced cross-presentation is mediated by mature DCs, is independent of endosomal acidification, and relies on cytosolic Ag processing machinery.

Original languageEnglish
Pages (from-to)4102-4110
Number of pages9
JournalJournal of Immunology
Volume170
Issue number8
StatePublished - Apr 15 2003

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Cross-Priming
Toll-Like Receptors
Dendritic Cells
Bone Marrow
Ligands
Cytidine Monophosphate
Inosine Monophosphate
Adaptive Immunity
T-Lymphocytes
DNA

ASJC Scopus subject areas

  • Immunology

Cite this

Datta, S. K., Redecke, V., Prilliman, K. R., Takabayashi, K., Corr, M., Tallant, T., ... Raz, E. (2003). A subset of toll-like receptor ligands induces cross-presentation by bone marrow-derived dendritic cells. Journal of Immunology, 170(8), 4102-4110.

A subset of toll-like receptor ligands induces cross-presentation by bone marrow-derived dendritic cells. / Datta, Sandip K.; Redecke, Vanessa; Prilliman, Kiley R.; Takabayashi, Kenji; Corr, Maripat; Tallant, Thomas; DiDonato, Joseph; Dziarski, Roman; Akira, Shizuo; Schoenberger, Stephen P.; Raz, Eyal.

In: Journal of Immunology, Vol. 170, No. 8, 15.04.2003, p. 4102-4110.

Research output: Contribution to journalArticle

Datta, SK, Redecke, V, Prilliman, KR, Takabayashi, K, Corr, M, Tallant, T, DiDonato, J, Dziarski, R, Akira, S, Schoenberger, SP & Raz, E 2003, 'A subset of toll-like receptor ligands induces cross-presentation by bone marrow-derived dendritic cells', Journal of Immunology, vol. 170, no. 8, pp. 4102-4110.
Datta SK, Redecke V, Prilliman KR, Takabayashi K, Corr M, Tallant T et al. A subset of toll-like receptor ligands induces cross-presentation by bone marrow-derived dendritic cells. Journal of Immunology. 2003 Apr 15;170(8):4102-4110.
Datta, Sandip K. ; Redecke, Vanessa ; Prilliman, Kiley R. ; Takabayashi, Kenji ; Corr, Maripat ; Tallant, Thomas ; DiDonato, Joseph ; Dziarski, Roman ; Akira, Shizuo ; Schoenberger, Stephen P. ; Raz, Eyal. / A subset of toll-like receptor ligands induces cross-presentation by bone marrow-derived dendritic cells. In: Journal of Immunology. 2003 ; Vol. 170, No. 8. pp. 4102-4110.
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