A translational bioinformatic approach in identifying and validating an interaction between Vitamin A and CYP19A1

Santosh Philips, Jing Zhou, Zhigao Li, Todd Skaar, Lang Li

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Introduction: One major challenge in personalized medicine research is to identify the environmental factors that can alter drug response, and to investigate their molecular mechanisms. These environmental factors include co-medications, food, and nutrition or dietary supplements. The increasing use of dietary supplements and their potential interactions with cytochrome P450 (CYP450) enzymes is a highly significant personalized medicine research domain, because most of the drugs on the market are metabolized through CYP450 enzymes. Methods: Initial bioinformatics analysis revealed a number of regulators of CYP450 enzymes from a human liver bank gene expression quantitative loci data set. Then, a compound-gene network was constructed from the curated literature data. This network consisted of compounds that interact with either CYPs and/or their regulators that influence either their gene expression or activity. We further evaluated this finding in three different cell lines: JEG3, HeLa, and LNCaP cells. Results: From a total of 868 interactions we were able to identify an interesting interaction between retinoic acid (i.e. Vitamin A) and the aromatase gene (i.e. CYP19A1). Our experimental results showed that retinoic acid at physiological concentration significantly influenced CYP19A1 gene expressions. Conclusions: These results suggest that the presence of retinoic acid may alter the efficacy of agents used to suppress aromatase expression.

Original languageEnglish (US)
Article numberS17
JournalBMC Genomics
Volume16
Issue number7
DOIs
StatePublished - Jun 11 2015

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Computational Biology
Vitamin A
Cytochrome P-450 Enzyme System
Dietary Supplements
Tretinoin
Precision Medicine
Aromatase
Gene Expression
Gene Regulatory Networks
HeLa Cells
Research
Pharmaceutical Preparations
Cell Line
Liver
Genes

ASJC Scopus subject areas

  • Biotechnology
  • Genetics

Cite this

A translational bioinformatic approach in identifying and validating an interaction between Vitamin A and CYP19A1. / Philips, Santosh; Zhou, Jing; Li, Zhigao; Skaar, Todd; Li, Lang.

In: BMC Genomics, Vol. 16, No. 7, S17, 11.06.2015.

Research output: Contribution to journalArticle

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