A unique pattern of expression of the four muscle regulatory factor proteins distinguishes somitic from embryonic, fetal and newborn mouse myogenic cells

Timothy H. Smith, Nancy E. Block, Simon Rhodes, Stephen F. Konieczny, Jeffrey Boone Miller

Research output: Contribution to journalArticle

98 Citations (Scopus)

Abstract

A unique pattern of expression of the four muscle regulatory factor (MRF) proteins was found to distinguish early somitic from embryonic, fetal and newborn limb myogenic cells in vitro. Expression of the myosin heavy chain (MHC), MyoD, myogenin, Myf-5, and MRF4 proteins was examined by immunocytochemistry in cultures of four distinct types of mouse myogenic cells: somitic (E8.5), embryonic (E11.5), fetal (E16.5) and newborn limb. In embryonic, fetal and newborn cultures, the MRF proteins were expressed in generally similar patterns: MyoD was the first MRF expressed; MyoD and myogenin were expressed by more cells than Myf-5 or MRF4; and each of the four MRFs was found both in cells that expressed MHC and in cells that did not express MHC. In cultures of somitic cells, in contrast, Myf-5 was expressed first and by more cells than MyoD or myogenin; MRF4 was not detected; and the MRFs were never found to be coexpressed with MHC in the same cell. Thus, some somitic cells had the unexpected ability to maintain MHC expression in the absence of detectable MRF protein expression. The different myogenic programs of embryonic, fetal and newborn myogenic cells are not, therefore, a simple result of qualitatively different MRF expression patterns, whereas myogenesis by somitic cells does include a unique pattern of MRF expression.

Original languageEnglish (US)
Pages (from-to)1125-1133
Number of pages9
JournalDevelopment (Cambridge)
Volume117
Issue number3
StatePublished - Mar 1993
Externally publishedYes

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Muscles
Myosin Heavy Chains
Myogenin
Proteins
Extremities
Muscle Development
Cell Culture Techniques
Immunohistochemistry
myogenic factor 6

Keywords

  • Mouse
  • Muscle regulatory factors
  • Myoblasts
  • Myogenesis
  • Somites

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy

Cite this

A unique pattern of expression of the four muscle regulatory factor proteins distinguishes somitic from embryonic, fetal and newborn mouse myogenic cells. / Smith, Timothy H.; Block, Nancy E.; Rhodes, Simon; Konieczny, Stephen F.; Miller, Jeffrey Boone.

In: Development (Cambridge), Vol. 117, No. 3, 03.1993, p. 1125-1133.

Research output: Contribution to journalArticle

Smith, Timothy H. ; Block, Nancy E. ; Rhodes, Simon ; Konieczny, Stephen F. ; Miller, Jeffrey Boone. / A unique pattern of expression of the four muscle regulatory factor proteins distinguishes somitic from embryonic, fetal and newborn mouse myogenic cells. In: Development (Cambridge). 1993 ; Vol. 117, No. 3. pp. 1125-1133.
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