A variant epithelial sub-population in normal thyroid with high proliferative capacity in vitro

J. A. Bond, F. S. Wyllie, Mircea Ivan, T. Dawson, D. Wynford-Thomas

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We describe the existence in normal human primary thyroid cultures of a hitherto unrecognised sub-population of epithelial cells. This variant phenotype is characterised by squamoid morphology, absence of thyroglobulin, and an altered profile of intermediate filament expression. We suggest that these cells are derived from scattered foci of squamous metaplasia present in the normal gland. Although they are initially present at a frequency of less than 10--4, their very high proliferative capacity enables them to outgrow the 'classical' follicular cells and confers a much increased capacity for gene transduction. Recognition of these cells is therefore crucial in the interpretation of long-term thyroid culture experiments and those involving in vitro gene transfer.

Original languageEnglish (US)
Pages (from-to)175-183
Number of pages9
JournalMolecular and Cellular Endocrinology
Volume93
Issue number2
DOIs
StatePublished - 1993
Externally publishedYes

Fingerprint

Gene transfer
Thyroglobulin
Thyroid Gland
Genes
Population
Intermediate Filaments
Experiments
Metaplasia
Epithelial Cells
Phenotype
In Vitro Techniques

Keywords

  • Cytokeratin
  • Follicular cell
  • Intermediate filament
  • Metaplasia

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

A variant epithelial sub-population in normal thyroid with high proliferative capacity in vitro. / Bond, J. A.; Wyllie, F. S.; Ivan, Mircea; Dawson, T.; Wynford-Thomas, D.

In: Molecular and Cellular Endocrinology, Vol. 93, No. 2, 1993, p. 175-183.

Research output: Contribution to journalArticle

Bond, J. A. ; Wyllie, F. S. ; Ivan, Mircea ; Dawson, T. ; Wynford-Thomas, D. / A variant epithelial sub-population in normal thyroid with high proliferative capacity in vitro. In: Molecular and Cellular Endocrinology. 1993 ; Vol. 93, No. 2. pp. 175-183.
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