A YAC Contig Spanning the Hypophosphatemic Rickets Disease Gene (HYP) Candidate Region

Fiona Francis, Peter S.N. Rowe, Michael J. Econs, Chee Gee See, Frances Benham, Jeffrey L.H. O'Riordan, Marc K. Drezner, Renata M.J. Hamvas, Hans Lehrach

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Abstract

Dominant X-linked hypophosphatemic rickets (HYP) is the most common form of familial rickets. Linkage studies have localized the gene for this disorder to Xp22.1 between the markers DXS365 and DXS274, a region estimated to be approximately 3.5 cM. We have constructed a 1.5-Mb YAC contig encompassing this region by hybridization screening of high-density YAC clone filters. Rapid chromosome walking was achieved by direct hybridization of a pool of Alu -PCR products derived from a YAC containing DXS365 to the filter grids. Overlaps between YACs in the contig were estimated by hybridization of end probes to YAC digest blots and by analysis of cosmid fingerprints obtained by hybridization of YAC inserts to a flow-sorted chromosome X cosmid library. All YACs in the contig have been verified by fluorescence in situ hybridization. Several YACs spanning the HYP gene candidate region were selected for further analysis by rare-cutter enzyme digestion and pulsed-field gel electrophoresis. We estimate that the markers flanking the disease region, DXS365 and DXS274, are less than 1 Mb apart. This clone contig map provides an essential resource for the isolation of the HYP gene.

Original languageEnglish (US)
Pages (from-to)229-237
Number of pages9
JournalGenomics
Volume21
Issue number1
DOIs
StatePublished - May 1 1994
Externally publishedYes

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ASJC Scopus subject areas

  • Genetics

Cite this

Francis, F., Rowe, P. S. N., Econs, M. J., See, C. G., Benham, F., O'Riordan, J. L. H., Drezner, M. K., Hamvas, R. M. J., & Lehrach, H. (1994). A YAC Contig Spanning the Hypophosphatemic Rickets Disease Gene (HYP) Candidate Region. Genomics, 21(1), 229-237. https://doi.org/10.1006/geno.1994.1247