A ZASP missense mutation, S196L, leads to cytoskeletal and electrical abnormalities in a mouse model of cardiomyopathy

Zhaohui Li, Tomohiko Ai, Kaveh Samani, Yutao Xi, Huei Ping Tzeng, Mingxing Xie, Shan Wu, Shuping Ge, Michael D. Taylor, Jian Wen Dong, Jie Cheng, Michael J. Ackerman, Akinori Kimura, Gianfranco Sinagra, Luca Brunelli, Georgine Faulkner, Matteo Vatta

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Background-Dilated cardiomyopathy (DCM) is a primary disease of the heart muscle associated with sudden cardiac death secondary to ventricular tachyarrhythmias and asystole. However, the molecular pathways linking DCM to arrhythmias and sudden cardiac death are unknown. We previously identified a S196L mutation in exon 4 of LBD3-encoded ZASP in a family with DCM and sudden cardiac death. These findings led us to hypothesize that this mutation may precipitate both cytoskeletal and conduction abnormalities in vivo. Therefore, we investigated the role of the ZASP4 mutation S196L in cardiac cytoarchitecture and ion channel biology. Methods and Results-We generated and analyzed transgenic mice with cardiac-restricted expression of the S196L mutation. We also performed cellular electrophysiological analysis on isolated S196L cardiomyocytes and proteinprotein interaction studies. Ten month-old S196L mice developed hemodynamic dysfunction consistent with DCM, whereas 3-month-old S196L mice presented with cardiac conduction defects and atrioventricular block. Electrophysiological analysis on isolated S196L cardiomyocytes demonstrated that the L-type Ca2+ currents and Na+ currents were altered. The pull-down assay demonstrated that ZASP4 complexes with both calcium (Ca v1.2) and sodium (Nav1.5) channels. Conclusions-Our findings provide new insight into the mechanisms by which mutations of a structural/cytoskeletal protein, such as ZASP, lead to cardiac functional and electric abnormalities. This work represents a novel framework to understand the development of conduction defects and arrhythmias in subjects with cardiomyopathies, including DCM.

Original languageEnglish (US)
Pages (from-to)646-656
Number of pages11
JournalCirculation: Arrhythmia and Electrophysiology
Volume3
Issue number6
DOIs
StatePublished - Dec 2010

Keywords

  • ACTN2
  • Arrhythmias
  • Ca1.2
  • Conduction
  • DCM
  • Na1.5
  • Telethonin
  • ZASP

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)
  • Medicine(all)

Fingerprint Dive into the research topics of 'A ZASP missense mutation, S196L, leads to cytoskeletal and electrical abnormalities in a mouse model of cardiomyopathy'. Together they form a unique fingerprint.

  • Cite this

    Li, Z., Ai, T., Samani, K., Xi, Y., Tzeng, H. P., Xie, M., Wu, S., Ge, S., Taylor, M. D., Dong, J. W., Cheng, J., Ackerman, M. J., Kimura, A., Sinagra, G., Brunelli, L., Faulkner, G., & Vatta, M. (2010). A ZASP missense mutation, S196L, leads to cytoskeletal and electrical abnormalities in a mouse model of cardiomyopathy. Circulation: Arrhythmia and Electrophysiology, 3(6), 646-656. https://doi.org/10.1161/CIRCEP.109.929240