Aberrant Expression of Neuropilin-1 and -2 in Human Pancreatic Cancer Cells

Kimi Fukahi, Mitsuharu Fukasawa, Gera Neufeld, Jun Itakura, Murray Korc

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Purpose: Neuropilin (Np)-1 and -2 are coreceptors for vascular endothelial growth factor (VEGF). This study was designed to assess their role in pancreatic ductal adenocarcinoma (PDAC). Experimental Design: We assessed Np-1 and Np-2 expression by real-time quantitative PCR in relation to the expression of VEGF ligands and receptors in pancreatic cancer cell lines and tissues. Results: ASPC-1, CAPAN-1, and PANC-1 pancreatic cancer cells and tumor-derived, laser-captured pancreatic cancer cells exhibited higher Np-1 and Np-2 mRNA levels than VEGF receptor-1, -2, or -3 mRNA levels. Transfection of Np-1 and Np-2 cDNAs in COS-7 cells, and treatment with tunicamycin revealed that both proteins were glycosylated. Both proteins were expressed in pancreatic cancer cell lines, in the PDAC samples, and in acinar cells adjacent to the cancer cells. The normal pancreas was devoid of Np-1 immunoreactivity, whereas Np-2 immunoreactivity was present in the endocrine islets and in some acinar cells, but not in ductal cells. Conclusions: The aberrant localization of Np-1 and Np-2 in the cancer cells in PDAC suggests that in addition to exerting proangiogenic effects, these coreceptors may contribute to novel autocrine-paracrine interactions in this malignancy.

Original languageEnglish (US)
Pages (from-to)581-590
Number of pages10
JournalClinical Cancer Research
Volume10
Issue number2
DOIs
StatePublished - Jan 15 2004
Externally publishedYes

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Neuropilin-2
Neuropilin-1
Pancreatic Neoplasms
Adenocarcinoma
Acinar Cells
Neoplasms
Vascular Endothelial Growth Factor Receptor-1
Tunicamycin
Cell Line
Vascular Endothelial Growth Factor Receptor-2
Messenger RNA
Vascular Endothelial Growth Factor Receptor
COS Cells
Vascular Endothelial Growth Factor A
Transfection
Real-Time Polymerase Chain Reaction
Pancreas
Proteins
Lasers
Research Design

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Aberrant Expression of Neuropilin-1 and -2 in Human Pancreatic Cancer Cells. / Fukahi, Kimi; Fukasawa, Mitsuharu; Neufeld, Gera; Itakura, Jun; Korc, Murray.

In: Clinical Cancer Research, Vol. 10, No. 2, 15.01.2004, p. 581-590.

Research output: Contribution to journalArticle

Fukahi, K, Fukasawa, M, Neufeld, G, Itakura, J & Korc, M 2004, 'Aberrant Expression of Neuropilin-1 and -2 in Human Pancreatic Cancer Cells', Clinical Cancer Research, vol. 10, no. 2, pp. 581-590. https://doi.org/10.1158/1078-0432.CCR-0930-03
Fukahi, Kimi ; Fukasawa, Mitsuharu ; Neufeld, Gera ; Itakura, Jun ; Korc, Murray. / Aberrant Expression of Neuropilin-1 and -2 in Human Pancreatic Cancer Cells. In: Clinical Cancer Research. 2004 ; Vol. 10, No. 2. pp. 581-590.
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