Aberrant expressions of AP-2α splice variants in pancreatic cancer

Catherine Carrière, Sarah Mirocha, Sophie Deharvengt, Jason R. Gunn, Murray Korc

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Objectives: The present study was conducted to evaluate the expression and function of AP-2α isoforms in pancreatic ductal adenocarcinoma. Methods: The expression of AP-2α was evaluated at the RNA level by reverse transcription-polymerase chain reaction and at the protein level by Western blotting and immunofluorescence. Its function as a transcription factor was evaluated in transient transfection experiments: DNA binding properties by electromobility shift assay and transactivation capabilities by luciferase assay. Results: Multiple alternative splicing events of AP-2α messenger occurred in all human pancreatic cancer cell lines, including a novel isoform, termed variant 6, which was not present in HeLa cells. At the protein level, except for 1 cell line, all pancreatic cancer cell lines expressed high nuclear levels of AP-2α. We also showed that AP-2α expressed by the pancreatic cancer cell lines could bind its cognate recognition site and activate transcription. However, variant 6, although not able to activate transcription, did not act in a dominant negative manner when cotransfected with the full-length protein. Conclusions: Multiple isoforms of AP-2α are highly expressed in pancreatic cancer cell lines including a new isoform, AP-2α variant 6, which seems to be pancreatic cancer specific and is deprived of transcriptional activity.

Original languageEnglish (US)
Pages (from-to)695-700
Number of pages6
JournalPancreas
Volume40
Issue number5
DOIs
StatePublished - Jul 1 2011

Keywords

  • AP-2α isoforms
  • Pancreatic ductal adenocarcinoma

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Hepatology
  • Endocrinology

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  • Cite this

    Carrière, C., Mirocha, S., Deharvengt, S., Gunn, J. R., & Korc, M. (2011). Aberrant expressions of AP-2α splice variants in pancreatic cancer. Pancreas, 40(5), 695-700. https://doi.org/10.1097/MPA.0b013e31821f2715