Abnormal expression of matrix metalloproteinases and tissue inhibitors of metalloproteinases in brain arteriovenous malformations

Tomoki Hashimoto, Gen Wen, Michael T. Lawton, Nancy J. Boudreau, Andrew W. Bollen, Guo Yuan Yang, Nicholas Barbaro, Randall T. Higashida, Christopher F. Dowd, Van V. Halbach, William L. Young

Research output: Contribution to journalArticle

99 Citations (Scopus)

Abstract

Background and Purpose - Excessive degradation of the vascular matrix by matrix metalloproteinases (MMPs) can lead to structural instability of vessels. In this study we examined the expression of MMPs and tissue inhibitors of metalloproteinases (TIMPs) in brain arteriovenous malformations (BAVMs). Methods - We performed gelatin zymography for MMPs and Western blot for MMP-9, MMP-2, TIMP-1, TIMP-2, TIMP-3, and TIMP-4. MMP-9 expression was localized by immunohistochemistry. Results - We analyzed 37 BAVM specimens and 9 control brain specimens from epilepsy surgery. Thirty-two BAVM patients had embolization treatment before resection. Eighteen BAVM patients had a history of hemorrhage from BAVMs. Neither MMP-2 nor TIMP-2 was detected in BAVMs or control brain specimens. Compared with control brain samples, BAVM samples had higher levels of total MMP-9, active MMP-9, pro-MMP-9, TIMP-1, and TIMP-3. TIMP-4 levels were higher in the control brain than in BAVM specimens. MMP-9 was localized to the endothelial cell/peri-endothelial cell layer and infiltrating neutrophils of BAVMs. BAVMs with venous stenosis ≥50% had higher expression of MMP-9 than BAVMs with venous stenosis

Original languageEnglish (US)
Pages (from-to)925-930
Number of pages6
JournalStroke
Volume34
Issue number4
DOIs
StatePublished - Apr 1 2003
Externally publishedYes

Fingerprint

Tissue Inhibitor of Metalloproteinases
Matrix Metalloproteinase Inhibitors
Arteriovenous Malformations
Brain
Matrix Metalloproteinase 9
Tissue Inhibitor of Metalloproteinase-3
Tissue Inhibitor of Metalloproteinase-2
Tissue Inhibitor of Metalloproteinase-1
Matrix Metalloproteinase 2
Matrix Metalloproteinases
Pathologic Constriction
Endothelial Cells
Intracranial Hemorrhages
Gelatin

Keywords

  • Angiogenesis
  • Cerebral aneurysm
  • Cerebral arteriovenous malformations
  • Subarachnoid hemorrhage
  • Vascular diseases

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Neuroscience(all)

Cite this

Hashimoto, T., Wen, G., Lawton, M. T., Boudreau, N. J., Bollen, A. W., Yang, G. Y., ... Young, W. L. (2003). Abnormal expression of matrix metalloproteinases and tissue inhibitors of metalloproteinases in brain arteriovenous malformations. Stroke, 34(4), 925-930. https://doi.org/10.1161/01.STR.0000061888.71524.DF

Abnormal expression of matrix metalloproteinases and tissue inhibitors of metalloproteinases in brain arteriovenous malformations. / Hashimoto, Tomoki; Wen, Gen; Lawton, Michael T.; Boudreau, Nancy J.; Bollen, Andrew W.; Yang, Guo Yuan; Barbaro, Nicholas; Higashida, Randall T.; Dowd, Christopher F.; Halbach, Van V.; Young, William L.

In: Stroke, Vol. 34, No. 4, 01.04.2003, p. 925-930.

Research output: Contribution to journalArticle

Hashimoto, T, Wen, G, Lawton, MT, Boudreau, NJ, Bollen, AW, Yang, GY, Barbaro, N, Higashida, RT, Dowd, CF, Halbach, VV & Young, WL 2003, 'Abnormal expression of matrix metalloproteinases and tissue inhibitors of metalloproteinases in brain arteriovenous malformations', Stroke, vol. 34, no. 4, pp. 925-930. https://doi.org/10.1161/01.STR.0000061888.71524.DF
Hashimoto, Tomoki ; Wen, Gen ; Lawton, Michael T. ; Boudreau, Nancy J. ; Bollen, Andrew W. ; Yang, Guo Yuan ; Barbaro, Nicholas ; Higashida, Randall T. ; Dowd, Christopher F. ; Halbach, Van V. ; Young, William L. / Abnormal expression of matrix metalloproteinases and tissue inhibitors of metalloproteinases in brain arteriovenous malformations. In: Stroke. 2003 ; Vol. 34, No. 4. pp. 925-930.
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AU - Wen, Gen

AU - Lawton, Michael T.

AU - Boudreau, Nancy J.

AU - Bollen, Andrew W.

AU - Yang, Guo Yuan

AU - Barbaro, Nicholas

AU - Higashida, Randall T.

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AU - Halbach, Van V.

AU - Young, William L.

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