Abnormalities in proinsulin processing in islets from individuals with longstanding T1D

Emily K. Sims, Farooq Syed, Julius Nyalwidhe, Henry T. Bahnson, Leena Haataja, Cate Speake, Margaret A. Morris, Appakalai N. Balamurugan, Raghavendra G. Mirmira, Jerry Nadler, Teresa L. Mastracci, Peter Arvan, Carla J. Greenbaum, Carmella Evans-Molina

Research output: Contribution to journalArticle

3 Scopus citations


We recently described the persistence of detectable serum proinsulin in a large majority of individuals with longstanding type 1 diabetes (T1D), including individuals with undetectable serum C-peptide. Here, we sought to further explore the mechanistic etiologies of persistent proinsulin secretion in T1D at the level of the islet, using tissues obtained from human donors. Immunostaining for proinsulin and insulin was performed on human pancreatic sections from the Network for Pancreatic Organ Donors with Diabetes (nPOD) collection (n = 24). Differential proinsulin processing enzyme expression was analyzed using mass spectrometry analysis of human islets isolated from pancreatic sections with laser capture microdissection (n = 6). Proinsulin processing enzyme mRNA levels were assessed using quantitative real-time PCR in isolated human islets (n = 10) treated with or without inflammatory cytokines. Compared to nondiabetic controls, immunostaining among a subset (4/9) of insulin positive T1D donor islets revealed increased numbers of cells with proinsulin-enriched, insulin-poor staining. T1D donor islets also exhibited increased proinsulin fluorescence intensity relative to insulin fluorescence intensity. Laser capture microdissection followed by mass spectrometry revealed reductions in the proinsulin processing enzymes prohormone convertase 1/3 (PC1/3) and carboxypeptidase E (CPE) in T1D donors. Twenty-four hour treatment of human islets with inflammatory cytokines reduced mRNA expression of the processing enzymes PC1/3, PC2, and CPE. Taken together, these data provide new mechanistic insight into altered proinsulin processing in long-duration T1D and suggest that reduced β cell prohormone processing is associated with proinflammatory cytokine-induced reductions in proinsulin processing enzyme expression.

Original languageEnglish (US)
Pages (from-to)90-99
Number of pages10
JournalTranslational Research
StatePublished - Nov 2019


  • CPE = carboxypeptidase E
  • ER = endoplasmic reticulum
  • IR = infrared
  • LCM = laser capture microdissection
  • LFQ = label-free quantification
  • PC1/3 = prohormone convertase 1/3
  • PC2 = prohormone convertase 2
  • T1D = type 1 diabetes
  • UV = ultraviolet
  • nPOD = network for pancreatic organ donors with type 1 diabetes

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Biochemistry, medical
  • Physiology (medical)

Fingerprint Dive into the research topics of 'Abnormalities in proinsulin processing in islets from individuals with longstanding T1D'. Together they form a unique fingerprint.

Cite this