Absence of erythrogenesis and vasculogenesis in Plcg1-deficient mice

Hong Jun Liao, Tsutomu Kume, Catriona McKay, Ming Jiang Xu, James N. Ihle, Graham Carpenter

Research output: Contribution to journalArticle

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Abstract

Mice nullizygous for Plcg1 cease growing at early to mid-gestation. An examination of carefully preserved wild-type embryos shows clear evidence of erythropoiesis, but erythropoiesis is not evident in Plcg1 nullizygous embryos at the same stage. The analyses of embryonic materials demonstrate that in the absence of Plcg1, erythroid progenitors cannot be detected in the yolk sac or embryo body by three different assays, burst-forming units, colony-forming units, and analysis for the developmental marker Ter119. However, non-erythroid granulocyte/macrophage colonies are produced by Plcg1 null embryos. Further analysis of these embryos demonstrates significantly diminished vasculogenesis in Plcg1 nullizygous embryos based on the lack of expression of the endothelial marker platelet endothelial cell adhesion molecule-1. In addition, Plcg1 nullizygous embryos express a greatly reduced level of vascular endothelial growth factor receptor-2/Flk-1, consistent with significantly impaired vasculogenesis and erythropoiesis. Interestingly, these early embryos do express phospholipase C-γ2, however, it is unable to substitute for the absence of phospholipase C-γ1, which can be detected in its tyrosine-phosphorylated state.

Original languageEnglish (US)
Pages (from-to)9335-9341
Number of pages7
JournalJournal of Biological Chemistry
Volume277
Issue number11
DOIs
StatePublished - Mar 15 2002
Externally publishedYes

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Type C Phospholipases
Embryonic Structures
CD31 Antigens
Vascular Endothelial Growth Factor Receptor-2
Macrophages
Tyrosine
Erythropoiesis
Assays
Yolk Sac
Granulocytes
Stem Cells
Pregnancy

ASJC Scopus subject areas

  • Biochemistry

Cite this

Liao, H. J., Kume, T., McKay, C., Xu, M. J., Ihle, J. N., & Carpenter, G. (2002). Absence of erythrogenesis and vasculogenesis in Plcg1-deficient mice. Journal of Biological Chemistry, 277(11), 9335-9341. https://doi.org/10.1074/jbc.M109955200

Absence of erythrogenesis and vasculogenesis in Plcg1-deficient mice. / Liao, Hong Jun; Kume, Tsutomu; McKay, Catriona; Xu, Ming Jiang; Ihle, James N.; Carpenter, Graham.

In: Journal of Biological Chemistry, Vol. 277, No. 11, 15.03.2002, p. 9335-9341.

Research output: Contribution to journalArticle

Liao, HJ, Kume, T, McKay, C, Xu, MJ, Ihle, JN & Carpenter, G 2002, 'Absence of erythrogenesis and vasculogenesis in Plcg1-deficient mice', Journal of Biological Chemistry, vol. 277, no. 11, pp. 9335-9341. https://doi.org/10.1074/jbc.M109955200
Liao, Hong Jun ; Kume, Tsutomu ; McKay, Catriona ; Xu, Ming Jiang ; Ihle, James N. ; Carpenter, Graham. / Absence of erythrogenesis and vasculogenesis in Plcg1-deficient mice. In: Journal of Biological Chemistry. 2002 ; Vol. 277, No. 11. pp. 9335-9341.
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