Modifications of existing techniques alowed the isolation of plasmid-like material from three cariogenic strains of Streptococcus mutans previously reported not to contain plasmids, but not from a noncariogenic, nonadherent, glucosyltransferase-defective mutant of this organism. This material was also isolated from four other cariogenic strains known to contain plasmids. Polyethylene glycol was used to precipitate this material from sodium dodecyl sulfate lysates of glycine, lysozyme and protease-treated spheroplasts. The material was not observed when polyethylene glycol was omitted from the procedure or when DNase was added. These modifications may provide a more sensitive method for the detection of plasmids than that used in other studies.
|Original language||English (US)|
|Number of pages||2|
|Journal||IRCS Medical Science|
|State||Published - Jan 1 1983|
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)