Absence of responsiveness to interferon-γ but not to interleukin 2 and depressed recycling as natural killer cell abnormalities in childhood lymphohistiocytic syndrome

F. C. Yang, A. Yabuhara, K. Koike

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Abstract

To characterize the abnormalities of natural killer (NK) cells in childhood lymphohistiocytic syndrome (LHS), we investigated the number and cytolytic functions of circulating NK cells in 10 LHS children using flow cytometry, 51Cr-release and single-cell assays. In the active phase, the numbers of CD16+ or CD56+ cells and NK activity were normal in more than half of the patients or otherwise decreased. Despite the treatment with interferon-γ (IFN-γ) there was no significant increase in NK activity in the children with LHS: the values (mean ± S.D.) of 32.2% ± 14.2% became 35.0% ± 13.3% (P > 0.05) when the control values changed from 45.5% ± 8.5% to 54.2% ± 10.1% after the stimulation. However, the NK cells normally responded to interleukin 2 (IL-2). In contrast, NK cells from 9 patients with infectious mononucleosis (IM) responded well to both IFN-γ and IL-2 (P <0.01). At the single-cell level, their NK cells had defective recycling capacity with normal killing capacity. The maximal recycling capacity (MRC) values (mean ± S.D.) were 3.6 ± 0.8 as compared to the control value of 5.5 ± 0.9 (P <0.01). Two of the patients studied had extremely high levels of serum IFN-γ (9.8 U/ml and 158.0 U/ml) as compared to the control value of

Original languageEnglish (US)
Pages (from-to)273-280
Number of pages8
JournalInternational Journal of Hematology
Volume59
Issue number4
StatePublished - 1994
Externally publishedYes

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Recycling
Natural Killer Cells
Interferons
Interleukin-2
Infectious Mononucleosis
Population Growth
Flow Cytometry
Serum

Keywords

  • IFN-γ
  • IL-2
  • LHS
  • NK activity

ASJC Scopus subject areas

  • Hematology

Cite this

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title = "Absence of responsiveness to interferon-γ but not to interleukin 2 and depressed recycling as natural killer cell abnormalities in childhood lymphohistiocytic syndrome",
abstract = "To characterize the abnormalities of natural killer (NK) cells in childhood lymphohistiocytic syndrome (LHS), we investigated the number and cytolytic functions of circulating NK cells in 10 LHS children using flow cytometry, 51Cr-release and single-cell assays. In the active phase, the numbers of CD16+ or CD56+ cells and NK activity were normal in more than half of the patients or otherwise decreased. Despite the treatment with interferon-γ (IFN-γ) there was no significant increase in NK activity in the children with LHS: the values (mean ± S.D.) of 32.2{\%} ± 14.2{\%} became 35.0{\%} ± 13.3{\%} (P > 0.05) when the control values changed from 45.5{\%} ± 8.5{\%} to 54.2{\%} ± 10.1{\%} after the stimulation. However, the NK cells normally responded to interleukin 2 (IL-2). In contrast, NK cells from 9 patients with infectious mononucleosis (IM) responded well to both IFN-γ and IL-2 (P <0.01). At the single-cell level, their NK cells had defective recycling capacity with normal killing capacity. The maximal recycling capacity (MRC) values (mean ± S.D.) were 3.6 ± 0.8 as compared to the control value of 5.5 ± 0.9 (P <0.01). Two of the patients studied had extremely high levels of serum IFN-γ (9.8 U/ml and 158.0 U/ml) as compared to the control value of",
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AU - Yabuhara, A.

AU - Koike, K.

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N2 - To characterize the abnormalities of natural killer (NK) cells in childhood lymphohistiocytic syndrome (LHS), we investigated the number and cytolytic functions of circulating NK cells in 10 LHS children using flow cytometry, 51Cr-release and single-cell assays. In the active phase, the numbers of CD16+ or CD56+ cells and NK activity were normal in more than half of the patients or otherwise decreased. Despite the treatment with interferon-γ (IFN-γ) there was no significant increase in NK activity in the children with LHS: the values (mean ± S.D.) of 32.2% ± 14.2% became 35.0% ± 13.3% (P > 0.05) when the control values changed from 45.5% ± 8.5% to 54.2% ± 10.1% after the stimulation. However, the NK cells normally responded to interleukin 2 (IL-2). In contrast, NK cells from 9 patients with infectious mononucleosis (IM) responded well to both IFN-γ and IL-2 (P <0.01). At the single-cell level, their NK cells had defective recycling capacity with normal killing capacity. The maximal recycling capacity (MRC) values (mean ± S.D.) were 3.6 ± 0.8 as compared to the control value of 5.5 ± 0.9 (P <0.01). Two of the patients studied had extremely high levels of serum IFN-γ (9.8 U/ml and 158.0 U/ml) as compared to the control value of

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