Accurate single-day titration of adenovirus vectors based on equivalence of protein VII nuclear dots and infectious particles

Marcin P. Walkiewicz, Nuria Morral, Daniel A. Engel

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Protein VII is an abundant component of adenovirus particles and is tightly associated with the viral DNA. It enters the nucleus along with the infecting viral genome and remains bound throughout early phase. Protein VII can be visualized by immunofluorescent staining as discrete dots in the infected cell nucleus. Comparison between protein VII staining and expression of the 72 kDa DNA-binding protein revealed a one-to-one correspondence between protein VII dots and infectious viral genomes. A similar relationship was observed for a helper-dependent adenovirus vector expressing green fluorescent protein. This relationship allowed accurate titration of adenovirus preparations, including wild-type and helper-dependent vectors, using a 1-day immunofluorescence method. The method can be applied to any adenovirus vector and gives results equivalent to the standard plaque assay.

Original languageEnglish
Pages (from-to)251-258
Number of pages8
JournalJournal of Virological Methods
Volume159
Issue number2
DOIs
StatePublished - Aug 2009

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Nuclear Proteins
Adenoviridae
Viral Genome
Proteins
Staining and Labeling
Viral DNA
DNA-Binding Proteins
Green Fluorescent Proteins
Cell Nucleus
Fluorescent Antibody Technique

Keywords

  • Adenovirus
  • Adenovirus vectors
  • Gene therapy
  • Protein VII
  • Viral titer

ASJC Scopus subject areas

  • Virology

Cite this

Accurate single-day titration of adenovirus vectors based on equivalence of protein VII nuclear dots and infectious particles. / Walkiewicz, Marcin P.; Morral, Nuria; Engel, Daniel A.

In: Journal of Virological Methods, Vol. 159, No. 2, 08.2009, p. 251-258.

Research output: Contribution to journalArticle

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