Acetylation of conserved lysines in bovine papillomavirus E2 by p300

Edward J. Quinlan, Sara P. Culleton, Shwu Yuan Wu, Cheng Ming Chiang, Elliot Androphy

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The p300, CBP, and pCAF lysine acetyltransferase (KAT) proteins have been reported to physically interact with bovine (BPV) and human (HPV) papillomavirus E2 proteins. While overexpression of these KAT proteins enhances E2-dependent transcription, the mechanism has not been determined. Using RNA interference (RNAi) to deplete these factors, we demonstrated that E2 transcriptional activity requires physiological levels of p300, CBP, and pCAF. Each protein appears to have a unique function in E2-dependent transcription, since overexpression of one KAT failed to compensate for RNAi knockdown of another KAT. Using an in vitro acetylation assay, we identified highly conserved lysines that are targeted by p300 for acetylation. The conservative changes of lysines at positions 111 and 112 to arginine were of particular interest. The K111R and the K111R/K112R mutants showed reduced transcriptional activity that was not responsive to p300 overexpression, while the K112R mutant retained activity. p300 and CBP were detected at the viral promoter; however, pCAF was not. We propose a model by which E2 transcriptional activity is controlled by p300-mediated acetylation of lysine 111. This model represents a novel mechanism regulating papillomavirus gene expression.

Original languageEnglish
Pages (from-to)1497-1507
Number of pages11
JournalJournal of Virology
Volume87
Issue number3
DOIs
StatePublished - Feb 2013

Fingerprint

Bovine papillomavirus
acetylation
Acetylation
Lysine
lysine
RNA Interference
Papillomaviridae
RNA interference
Proteins
proteins
transcription (genetics)
mutants
acetyltransferases
Arginine
arginine
Gene Expression
promoter regions
gene expression
cattle
assays

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Acetylation of conserved lysines in bovine papillomavirus E2 by p300. / Quinlan, Edward J.; Culleton, Sara P.; Wu, Shwu Yuan; Chiang, Cheng Ming; Androphy, Elliot.

In: Journal of Virology, Vol. 87, No. 3, 02.2013, p. 1497-1507.

Research output: Contribution to journalArticle

Quinlan, Edward J. ; Culleton, Sara P. ; Wu, Shwu Yuan ; Chiang, Cheng Ming ; Androphy, Elliot. / Acetylation of conserved lysines in bovine papillomavirus E2 by p300. In: Journal of Virology. 2013 ; Vol. 87, No. 3. pp. 1497-1507.
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