Achieving an early pregnancy following allogeneic uterine transplantation in a rabbit model

Srdjan Saso, Gemma Petts, Anna L. David, Meen Yau Thum, Jayanta Chatterjee, Jose S. Vicente, Francisco Marco-Jimenez, David Corless, Michael Boyd, David Noakes, Iain Lindsay, Giuseppe Del Priore, Sadaf Ghaem-Maghami, J. Richard Smith

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Objective Uterine transplantation (UTx) has been proposed as a treatment option for women diagnosed with absolute uterine factor infertility (AUFI). The goal of UTx remains achieving pregnancy and live birth of a healthy neonate following allogeneic UTx. Our aim was to assess whether fertility was possible following allogeneic uterine transplantation (UTx), when the recipient had demonstrated long-term survival and had been administered immunosuppression. Study design Nine allogeneic UTx in New Zealand White rabbits were performed using a pre-determined protocol. Tacrolimus was the immunosuppressant selected. Embryos were transferred into both cornua of the sole living recipient via a mini-midline laparotomy. The pregnancy was monitored with regular reproductive profiles and serial trans-abdominal ultrasound to measure conceptus growth (gestation sac and crown rump length (CRL)). Results In the sole surviving doe a gestation sac was visualised on ultrasound from Day 9 (D9) after embryo transfer. Gestation sac diameter and CRL increased from D9 to D16 but by D18 the gestation sac had reduced in size. The fetus was no longer visible, suggesting fetal resorption had occurred. Subsequent scans on D22 and D25 did not demonstrate a gestation sac. Scheduled necropsy on D27 and histopathology confirmed evidence of a gravid uterus and presence of a gestational sac. A single episode of acute rejection occurred on D13. Conclusion Pregnancy was achieved after rabbit allogeneic UTx but serial ultrasound suggested that fetal demise occurred prior to scheduled necropsy. The study represents only the third example of conception and pregnancy following an animal allogeneic UTx.

Original languageEnglish (US)
Pages (from-to)164-169
Number of pages6
JournalEuropean Journal of Obstetrics Gynecology and Reproductive Biology
Volume185
DOIs
StatePublished - Feb 1 2015
Externally publishedYes

Fingerprint

Gestational Sac
Homologous Transplantation
Rabbits
Pregnancy
Crown-Rump Length
Animal Pregnancy
Fetal Resorption
Fetal Death
Embryo Transfer
Live Birth
Tacrolimus
Immunosuppressive Agents
Laparotomy
Immunosuppression
Infertility
Uterus
Fertility
Fetus
Embryonic Structures
Transplantation

Keywords

  • Fertility
  • Pregnancy
  • Rabbit
  • Uterine transplantation

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Reproductive Medicine
  • Medicine(all)

Cite this

Achieving an early pregnancy following allogeneic uterine transplantation in a rabbit model. / Saso, Srdjan; Petts, Gemma; David, Anna L.; Thum, Meen Yau; Chatterjee, Jayanta; Vicente, Jose S.; Marco-Jimenez, Francisco; Corless, David; Boyd, Michael; Noakes, David; Lindsay, Iain; Del Priore, Giuseppe; Ghaem-Maghami, Sadaf; Smith, J. Richard.

In: European Journal of Obstetrics Gynecology and Reproductive Biology, Vol. 185, 01.02.2015, p. 164-169.

Research output: Contribution to journalArticle

Saso, S, Petts, G, David, AL, Thum, MY, Chatterjee, J, Vicente, JS, Marco-Jimenez, F, Corless, D, Boyd, M, Noakes, D, Lindsay, I, Del Priore, G, Ghaem-Maghami, S & Smith, JR 2015, 'Achieving an early pregnancy following allogeneic uterine transplantation in a rabbit model', European Journal of Obstetrics Gynecology and Reproductive Biology, vol. 185, pp. 164-169. https://doi.org/10.1016/j.ejogrb.2014.12.017
Saso, Srdjan ; Petts, Gemma ; David, Anna L. ; Thum, Meen Yau ; Chatterjee, Jayanta ; Vicente, Jose S. ; Marco-Jimenez, Francisco ; Corless, David ; Boyd, Michael ; Noakes, David ; Lindsay, Iain ; Del Priore, Giuseppe ; Ghaem-Maghami, Sadaf ; Smith, J. Richard. / Achieving an early pregnancy following allogeneic uterine transplantation in a rabbit model. In: European Journal of Obstetrics Gynecology and Reproductive Biology. 2015 ; Vol. 185. pp. 164-169.
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abstract = "Objective Uterine transplantation (UTx) has been proposed as a treatment option for women diagnosed with absolute uterine factor infertility (AUFI). The goal of UTx remains achieving pregnancy and live birth of a healthy neonate following allogeneic UTx. Our aim was to assess whether fertility was possible following allogeneic uterine transplantation (UTx), when the recipient had demonstrated long-term survival and had been administered immunosuppression. Study design Nine allogeneic UTx in New Zealand White rabbits were performed using a pre-determined protocol. Tacrolimus was the immunosuppressant selected. Embryos were transferred into both cornua of the sole living recipient via a mini-midline laparotomy. The pregnancy was monitored with regular reproductive profiles and serial trans-abdominal ultrasound to measure conceptus growth (gestation sac and crown rump length (CRL)). Results In the sole surviving doe a gestation sac was visualised on ultrasound from Day 9 (D9) after embryo transfer. Gestation sac diameter and CRL increased from D9 to D16 but by D18 the gestation sac had reduced in size. The fetus was no longer visible, suggesting fetal resorption had occurred. Subsequent scans on D22 and D25 did not demonstrate a gestation sac. Scheduled necropsy on D27 and histopathology confirmed evidence of a gravid uterus and presence of a gestational sac. A single episode of acute rejection occurred on D13. Conclusion Pregnancy was achieved after rabbit allogeneic UTx but serial ultrasound suggested that fetal demise occurred prior to scheduled necropsy. The study represents only the third example of conception and pregnancy following an animal allogeneic UTx.",
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AU - Chatterjee, Jayanta

AU - Vicente, Jose S.

AU - Marco-Jimenez, Francisco

AU - Corless, David

AU - Boyd, Michael

AU - Noakes, David

AU - Lindsay, Iain

AU - Del Priore, Giuseppe

AU - Ghaem-Maghami, Sadaf

AU - Smith, J. Richard

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N2 - Objective Uterine transplantation (UTx) has been proposed as a treatment option for women diagnosed with absolute uterine factor infertility (AUFI). The goal of UTx remains achieving pregnancy and live birth of a healthy neonate following allogeneic UTx. Our aim was to assess whether fertility was possible following allogeneic uterine transplantation (UTx), when the recipient had demonstrated long-term survival and had been administered immunosuppression. Study design Nine allogeneic UTx in New Zealand White rabbits were performed using a pre-determined protocol. Tacrolimus was the immunosuppressant selected. Embryos were transferred into both cornua of the sole living recipient via a mini-midline laparotomy. The pregnancy was monitored with regular reproductive profiles and serial trans-abdominal ultrasound to measure conceptus growth (gestation sac and crown rump length (CRL)). Results In the sole surviving doe a gestation sac was visualised on ultrasound from Day 9 (D9) after embryo transfer. Gestation sac diameter and CRL increased from D9 to D16 but by D18 the gestation sac had reduced in size. The fetus was no longer visible, suggesting fetal resorption had occurred. Subsequent scans on D22 and D25 did not demonstrate a gestation sac. Scheduled necropsy on D27 and histopathology confirmed evidence of a gravid uterus and presence of a gestational sac. A single episode of acute rejection occurred on D13. Conclusion Pregnancy was achieved after rabbit allogeneic UTx but serial ultrasound suggested that fetal demise occurred prior to scheduled necropsy. The study represents only the third example of conception and pregnancy following an animal allogeneic UTx.

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