Acicid isoferritin stimulates differentiation of normal granulomonocytic progenitors

J. E T E Guimaraes, J. J. Berney, Hal Broxmeyer, A. V. Hoffbrand, G. E. Francis

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Acidic isoferritin (AIF) has been shown to be released by cells from patients with leukemia and to have an inhibitory effect on the growth of normal granulomonocytic (GM) progenitors (leukemia-inhibitory activity) during the S phase of the cell cycle. AIF is also produced by normal mature cells of the monocytemacrophage lineage. We studied the effects of IAF on the differentiation of normal GM progenitors and found an increase in the number of mature cells in AIF-exposed cultures. This increase did not occur when AIF was pretreated with anti-heart ferritin antiserum or when basic isoferritin was used in the place of AIF. The influence of AIF was not mimicked by removing S phase cells by pretreatment with a pulse of high specific activity tritiated thymidine. Thus, the apparent differentiation-stimulating effect of AIF is not likely to be due to selective removal of immature dividing cells. The results suggest that AIF-inhibitory activity on the proliferation of GM progenitors might at least in part be mediated by a stimulus for differentiation of the target cells, thus regulating the number of mature cells which might be formed by a single progenitor cell.

Original languageEnglish (US)
Pages (from-to)466-471
Number of pages6
JournalLeukemia
Volume2
Issue number7
StatePublished - 1988
Externally publishedYes

Fingerprint

Ferritins
S Phase
Leukemia
Cell Count
acidic isoferritin
Cell Lineage
Thymidine
Pulse
Immune Sera
Cell Differentiation
Cell Cycle
Stem Cells
Growth

ASJC Scopus subject areas

  • Cancer Research
  • Hematology

Cite this

Guimaraes, J. E. T. E., Berney, J. J., Broxmeyer, H., Hoffbrand, A. V., & Francis, G. E. (1988). Acicid isoferritin stimulates differentiation of normal granulomonocytic progenitors. Leukemia, 2(7), 466-471.

Acicid isoferritin stimulates differentiation of normal granulomonocytic progenitors. / Guimaraes, J. E T E; Berney, J. J.; Broxmeyer, Hal; Hoffbrand, A. V.; Francis, G. E.

In: Leukemia, Vol. 2, No. 7, 1988, p. 466-471.

Research output: Contribution to journalArticle

Guimaraes, JETE, Berney, JJ, Broxmeyer, H, Hoffbrand, AV & Francis, GE 1988, 'Acicid isoferritin stimulates differentiation of normal granulomonocytic progenitors', Leukemia, vol. 2, no. 7, pp. 466-471.
Guimaraes JETE, Berney JJ, Broxmeyer H, Hoffbrand AV, Francis GE. Acicid isoferritin stimulates differentiation of normal granulomonocytic progenitors. Leukemia. 1988;2(7):466-471.
Guimaraes, J. E T E ; Berney, J. J. ; Broxmeyer, Hal ; Hoffbrand, A. V. ; Francis, G. E. / Acicid isoferritin stimulates differentiation of normal granulomonocytic progenitors. In: Leukemia. 1988 ; Vol. 2, No. 7. pp. 466-471.
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