Acidic isoferritins do not suppress differentiation of mouse myeloid leukemic M1 cells

Junko Okabe-Kado, Moriaki Hayashi, Yoshio Honma, Motoo Hozumi, Hal Broxmeyer

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The differentiation of mouse myeloid leukemia line cells (M1) into macrophages or granulocytes has been reported. Resistant M1 cells which did not differentiate even with a high concentration of inducer of differentiation were isolated from M1 cells. The conditioned medium of the resistant M1 cells (RCM) inhibited the induction of differentiation of M1 cells and the formation of macrophage and granulocyte colonies of normal mouse bone marrow cells. Acidic isoferritins known as negative regulators of normal bone marrow cells (CFU-GM) failed to inhibit the induction of differentiation and growth of M1 cells. The RCM treated with anti-acidic isoferritin serum could inhibit the induction of differentiation of M1 cells as did the untreated RCM. The activities, in RCM, inhibiting growth and differentiation of the normal bone marrow cells were partly neutralized by treatment with the antiserum but most of the activities remained. These results suggest that growth and differentiation of the mouse myeloid leukemia M1 cells are not regulated by acidic isoferritins and other inhibitory activities affecting normal bone marrow colony formation, in addition to acidic isoferritins, are released from M1 cells.

Original languageEnglish (US)
Pages (from-to)811-815
Number of pages5
JournalLeukemia Research
Volume7
Issue number6
DOIs
StatePublished - 1983
Externally publishedYes

Fingerprint

Bone Marrow Cells
Myeloid Leukemia
Granulocytes
Cell Differentiation
Growth
Macrophages
Granulocyte-Macrophage Progenitor Cells
Conditioned Culture Medium
acidic isoferritin
Immune Sera
Bone Marrow
Serum

Keywords

  • acidic isoferritins
  • differentiation
  • differentiation inhibiting factors
  • Myeloid leukemia cells

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology

Cite this

Acidic isoferritins do not suppress differentiation of mouse myeloid leukemic M1 cells. / Okabe-Kado, Junko; Hayashi, Moriaki; Honma, Yoshio; Hozumi, Motoo; Broxmeyer, Hal.

In: Leukemia Research, Vol. 7, No. 6, 1983, p. 811-815.

Research output: Contribution to journalArticle

Okabe-Kado, Junko ; Hayashi, Moriaki ; Honma, Yoshio ; Hozumi, Motoo ; Broxmeyer, Hal. / Acidic isoferritins do not suppress differentiation of mouse myeloid leukemic M1 cells. In: Leukemia Research. 1983 ; Vol. 7, No. 6. pp. 811-815.
@article{3688d706cf4f4efd9c6e60cfef3aacf4,
title = "Acidic isoferritins do not suppress differentiation of mouse myeloid leukemic M1 cells",
abstract = "The differentiation of mouse myeloid leukemia line cells (M1) into macrophages or granulocytes has been reported. Resistant M1 cells which did not differentiate even with a high concentration of inducer of differentiation were isolated from M1 cells. The conditioned medium of the resistant M1 cells (RCM) inhibited the induction of differentiation of M1 cells and the formation of macrophage and granulocyte colonies of normal mouse bone marrow cells. Acidic isoferritins known as negative regulators of normal bone marrow cells (CFU-GM) failed to inhibit the induction of differentiation and growth of M1 cells. The RCM treated with anti-acidic isoferritin serum could inhibit the induction of differentiation of M1 cells as did the untreated RCM. The activities, in RCM, inhibiting growth and differentiation of the normal bone marrow cells were partly neutralized by treatment with the antiserum but most of the activities remained. These results suggest that growth and differentiation of the mouse myeloid leukemia M1 cells are not regulated by acidic isoferritins and other inhibitory activities affecting normal bone marrow colony formation, in addition to acidic isoferritins, are released from M1 cells.",
keywords = "acidic isoferritins, differentiation, differentiation inhibiting factors, Myeloid leukemia cells",
author = "Junko Okabe-Kado and Moriaki Hayashi and Yoshio Honma and Motoo Hozumi and Hal Broxmeyer",
year = "1983",
doi = "10.1016/0145-2126(83)90075-9",
language = "English (US)",
volume = "7",
pages = "811--815",
journal = "Leukemia Research",
issn = "0145-2126",
publisher = "Elsevier Limited",
number = "6",

}

TY - JOUR

T1 - Acidic isoferritins do not suppress differentiation of mouse myeloid leukemic M1 cells

AU - Okabe-Kado, Junko

AU - Hayashi, Moriaki

AU - Honma, Yoshio

AU - Hozumi, Motoo

AU - Broxmeyer, Hal

PY - 1983

Y1 - 1983

N2 - The differentiation of mouse myeloid leukemia line cells (M1) into macrophages or granulocytes has been reported. Resistant M1 cells which did not differentiate even with a high concentration of inducer of differentiation were isolated from M1 cells. The conditioned medium of the resistant M1 cells (RCM) inhibited the induction of differentiation of M1 cells and the formation of macrophage and granulocyte colonies of normal mouse bone marrow cells. Acidic isoferritins known as negative regulators of normal bone marrow cells (CFU-GM) failed to inhibit the induction of differentiation and growth of M1 cells. The RCM treated with anti-acidic isoferritin serum could inhibit the induction of differentiation of M1 cells as did the untreated RCM. The activities, in RCM, inhibiting growth and differentiation of the normal bone marrow cells were partly neutralized by treatment with the antiserum but most of the activities remained. These results suggest that growth and differentiation of the mouse myeloid leukemia M1 cells are not regulated by acidic isoferritins and other inhibitory activities affecting normal bone marrow colony formation, in addition to acidic isoferritins, are released from M1 cells.

AB - The differentiation of mouse myeloid leukemia line cells (M1) into macrophages or granulocytes has been reported. Resistant M1 cells which did not differentiate even with a high concentration of inducer of differentiation were isolated from M1 cells. The conditioned medium of the resistant M1 cells (RCM) inhibited the induction of differentiation of M1 cells and the formation of macrophage and granulocyte colonies of normal mouse bone marrow cells. Acidic isoferritins known as negative regulators of normal bone marrow cells (CFU-GM) failed to inhibit the induction of differentiation and growth of M1 cells. The RCM treated with anti-acidic isoferritin serum could inhibit the induction of differentiation of M1 cells as did the untreated RCM. The activities, in RCM, inhibiting growth and differentiation of the normal bone marrow cells were partly neutralized by treatment with the antiserum but most of the activities remained. These results suggest that growth and differentiation of the mouse myeloid leukemia M1 cells are not regulated by acidic isoferritins and other inhibitory activities affecting normal bone marrow colony formation, in addition to acidic isoferritins, are released from M1 cells.

KW - acidic isoferritins

KW - differentiation

KW - differentiation inhibiting factors

KW - Myeloid leukemia cells

UR - http://www.scopus.com/inward/record.url?scp=0021017270&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021017270&partnerID=8YFLogxK

U2 - 10.1016/0145-2126(83)90075-9

DO - 10.1016/0145-2126(83)90075-9

M3 - Article

C2 - 6582344

AN - SCOPUS:0021017270

VL - 7

SP - 811

EP - 815

JO - Leukemia Research

JF - Leukemia Research

SN - 0145-2126

IS - 6

ER -