Actin depolymerization factor/cofilin activation regulates actin polymerization and tension development in canine tracheal smooth muscle

Rong Zhao, Liping Du, Youliang Huang, Yidi Wu, Susan Gunst

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

The contractile activation of airway smooth muscle tissues stimulates actin polymerization, and the inhibition of actin polymerization inhibits tension development. Actin-depolymerizing factor (ADF) and cofilin are members of a family of actin-binding proteins that mediate the severing of F-actin when activated by dephosphorylation at serine 3. The role of ADF/cofilin activation in the regulation of actin dynamics and tension development during the contractile activation of smooth muscle was evaluated in intact canine tracheal smooth muscle tissues. Two-dimensional gel electrophoresis revealed that ADF and cofilin exist in similar proportions in the muscle tissues, and that ∼40% of the total ADF/cofilin in unstimulated tissues is phosphorylated. Phospho-ADF/cofilin decreased concurrently with tension development in response to stimulation with acetylcholine (ACh) or potassium depolarization indicating the activation of ADF/cofilin. Expression of an inactive phospho-cofilin mimetic (cofilin S3E) but not wild type cofilin in the smooth muscle tissues inhibited endogenous ADF/cofilin dephosphorylation and ACh-induced actin polymerization. Expression of cofilin S3E in the tissues depressed tension development in response to ACh, but it did not affect myosin light chain phosphorylation. The ACh-induced dephosphorylation of ADF/cofilin required the Ca2+- dependent activation of calcineurin (PP2B). The results indicate that the activation of ADF/cofilin is regulated by contractile stimulation in tracheal smooth muscle and that cofilin activation is required for actin polymerization and tension development in response to contractile stimulation.

Original languageEnglish
Pages (from-to)36522-36531
Number of pages10
JournalJournal of Biological Chemistry
Volume283
Issue number52
DOIs
StatePublished - Dec 26 2008

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Actin Depolymerizing Factors
Depolymerization
Destrin
Polymerization
Smooth Muscle
Muscle
Canidae
Actins
Chemical activation
Tissue
Acetylcholine
Muscles
Cofilin 2
Microfilament Proteins
Myosin Light Chains
Phosphorylation
Calcineurin
Depolarization
Electrophoresis, Gel, Two-Dimensional
Electrophoresis

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Actin depolymerization factor/cofilin activation regulates actin polymerization and tension development in canine tracheal smooth muscle. / Zhao, Rong; Du, Liping; Huang, Youliang; Wu, Yidi; Gunst, Susan.

In: Journal of Biological Chemistry, Vol. 283, No. 52, 26.12.2008, p. 36522-36531.

Research output: Contribution to journalArticle

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