Action of fluorescein mercuric acetate upon mitochondrial energized processes

Martin J. Lee, Robert Harris, David E. Green

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Fluorescein mercuric acetate (FMA{black star} {black star} Abbreviations used in this paper are: m-ClCCP, carbonyl cyanide m-chlorophenyl hydrazone; FMA, fluorescein mercuric acetate; HBHM, heavy beef heart mitochondria; ETPH, phosphorylating submitochondrial particles; DNP, dinitrophenol; p-CMPS, p-chloromercuriphenylsulfonate; Pi, inorganic phosphate.) has been shown to be a highly selective reagent for suppressing the energized to energized-twisted configurational transition of heavy beef heart mitochondria as determined by electron microscopy and by measurement of light scattering changes. At the concentrations required for this selective suppression, FMA does not affect the primary conformational changes of energy coupling as measured by the proton shift reaction. Furthermore, it has been shown that FMA prevents the binding of inorganic phosphate by beef heart mitochondria in the energized state. The mechanism of action of FMA on oxidative phosphorylation and energized translocation of Ca++ can be rationalized in terms of an energy cycle involving two successive conformational changes - a primary energy-conserving conformational change (FMA-insensitive) resulting in proton release and cation uptake, and a secondary conformational change (FMA-sensitive) involving binding of inorganic phosphate.

Original languageEnglish (US)
Pages (from-to)937-946
Number of pages10
JournalBiochemical and Biophysical Research Communications
Volume36
Issue number6
StatePublished - Sep 10 1969
Externally publishedYes

Fingerprint

Heart Mitochondria
Beef
Mitochondria
Phosphates
Protons
4-Chloromercuribenzenesulfonate
Carbonyl Cyanide m-Chlorophenyl Hydrazone
Submitochondrial Particles
Dinitrophenols
Oxidative Phosphorylation
Light scattering
Electron microscopy
Stars
Cations
Electron Microscopy
Light
fluorescein mercuric acetate
Red Meat

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

Cite this

Action of fluorescein mercuric acetate upon mitochondrial energized processes. / Lee, Martin J.; Harris, Robert; Green, David E.

In: Biochemical and Biophysical Research Communications, Vol. 36, No. 6, 10.09.1969, p. 937-946.

Research output: Contribution to journalArticle

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