Action of secretagogues on a new preparation of functionally intact, isolated pancreatic acini.

J. A. Williams, Murray Korc, R. L. Dormer

Research output: Contribution to journalArticle

444 Citations (Scopus)

Abstract

Isolated pancreatic acini were prepared by a new method from mouse and rat pancreases by digestion with purified collagenase and chymotrypsin followed by mechanical shearing. Acini were structurally similar to those of the intact pancreas, having a normal luminal structure but with the basal acinar cell membranes exposed to the incubation medium. Amylase release in response to both cholinergic analogues and the cholecystokinin analogues caerulein and pentagastrin was comparable to that of the intact pancreas, but was much greater than previously reported for isolated acinar cells. Cholinergic-stimulated release was inhibited by atropine with a Ki value of 1.4 nM which is comparable to other muscarinic receptors. All agonists tested, when added at supramaximal concentrations, produced a submaximal release of amylase even though ATP levels and the release of slowly exchanging 45Ca2+ were normal or increased. Acini releasing amylase submaximally after being exposed to supramaximal concentrations of carbachol failed to respond to a maximal amount of caerulein or to the Ca2+ ionophore A23187. It is concluded that the decreased response (desensitization) is a postreceptor phenomenon and possibly mediated by Ca2+ itself.

Original languageEnglish
Pages (from-to)517-524
Number of pages8
JournalThe American journal of physiology
Volume235
Issue number5
StatePublished - Nov 1978
Externally publishedYes

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Amylases
Ceruletide
Pancreas
Acinar Cells
Cholinergic Agents
Pentagastrin
Cholecystokinin
Ionophores
Calcimycin
Chymotrypsin
Carbachol
Muscarinic Receptors
Collagenases
Atropine
Digestion
Adenosine Triphosphate
Cell Membrane

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Action of secretagogues on a new preparation of functionally intact, isolated pancreatic acini. / Williams, J. A.; Korc, Murray; Dormer, R. L.

In: The American journal of physiology, Vol. 235, No. 5, 11.1978, p. 517-524.

Research output: Contribution to journalArticle

Williams, J. A. ; Korc, Murray ; Dormer, R. L. / Action of secretagogues on a new preparation of functionally intact, isolated pancreatic acini. In: The American journal of physiology. 1978 ; Vol. 235, No. 5. pp. 517-524.
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