Activated K-Ras and INK4a/Arf deficiency promote aggressiveness of pancreatic cancer by induction of EMT consistent with cancer stem cell phenotype

Zhiwei Wang, Shadan Ali, Sanjeev Banerjee, Bin Bao, Yiwei Li, Asfar S. Azmi, Murray Korc, Fazlul H. Sarkar

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Pancreatic ductal adenocarcinoma (PDAC) is one of the most frequently diagnosed cancers and the fourth leading cause of cancer-related death in the United States, suggesting that there is an urgent need to design novel strategies for achieving better treatment outcome of patients diagnosed with PDAC. Our previous study has shown that activation of Notch and NF-κB play a critical role in the development of PDAC in the compound K-RasG12D and Ink4a/Arf deficient transgenic mice. However, the exact molecular mechanism by which mutated K-Ras and Ink4a/Arf deficiency contribute to progression of PDAC remains largely elusive. In the present study, we used multiple methods, such as real-time RT-PCR, Western blotting assay, and immunohistochemistry to gain further mechanistic insight. We found that the deletion of Ink4a/Arf in K-RasG12D expressing mice led to high expression of PDGF-D signaling pathway in the tumor and tumor-derived cell line (RInk-1 cells). Furthermore, PDGF-D knock-down in RInk-1 cells resulted in the inhibition of pancreatosphere formation and down-regulation of EZH2, CD44, EpCAM, and vimentin. Moreover, we demonstrated that epithelial-mesenchymal transition (EMT) was induced in the compound mice, which is linked with aggressiveness of PDAC. In addition, we demonstrated that tumors from compound transgenic mice have higher expression of cancer stem cell (CSC) markers. These results suggest that the acquisition of EMT phenotype and induction of CSC characteristics could be linked with the aggressiveness of PDAC mediated in part through the activation of PDGF-D, signaling.

Original languageEnglish
Pages (from-to)556-562
Number of pages7
JournalJournal of Cellular Physiology
Volume228
Issue number3
DOIs
StatePublished - Mar 2013

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Epithelial-Mesenchymal Transition
Neoplastic Stem Cells
Stem cells
Pancreatic Neoplasms
Tumors
Adenocarcinoma
Phenotype
Chemical activation
Vimentin
Transgenic Mice
Assays
Neoplasms
Cells
Tumor Cell Line
Real-Time Polymerase Chain Reaction
Down-Regulation
Western Blotting
Immunohistochemistry

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Activated K-Ras and INK4a/Arf deficiency promote aggressiveness of pancreatic cancer by induction of EMT consistent with cancer stem cell phenotype. / Wang, Zhiwei; Ali, Shadan; Banerjee, Sanjeev; Bao, Bin; Li, Yiwei; Azmi, Asfar S.; Korc, Murray; Sarkar, Fazlul H.

In: Journal of Cellular Physiology, Vol. 228, No. 3, 03.2013, p. 556-562.

Research output: Contribution to journalArticle

Wang, Zhiwei ; Ali, Shadan ; Banerjee, Sanjeev ; Bao, Bin ; Li, Yiwei ; Azmi, Asfar S. ; Korc, Murray ; Sarkar, Fazlul H. / Activated K-Ras and INK4a/Arf deficiency promote aggressiveness of pancreatic cancer by induction of EMT consistent with cancer stem cell phenotype. In: Journal of Cellular Physiology. 2013 ; Vol. 228, No. 3. pp. 556-562.
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AU - Bao, Bin

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AU - Azmi, Asfar S.

AU - Korc, Murray

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