Activation and routing of membrane-tethered prohormone convertases 1 and 2

Angela Bruzzaniti, Ruth Marx, Richard E. Mains

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Many peptide hormones and neuropeptides are processed by members of the subtilisin-like family of prohormone convertases (PCs), which are either soluble or integral membrane proteins. PC1 and PC2 are soluble PCs that are primarily localized to large dense core vesicles in neurons and endocrine cells. We examined whether PC1 and PC2 were active when expressed as membrane-tethered proteins, and how tethering to membranes alters the biosynthesis, enzymatic activity, and intracellular routing of these PCs. PC1 and PC2 chimeras were constructed using the transmembrane domain and cytoplasmic domain of the am/dating enzyme, peptidylglycine α-amidating monooxygenase (PAM). The membrane-tethered PCs were rerouted from large dense core vesicles to the Golgi region. In addition, the chimeras were transiently expressed at the cell surface and rapidly internalized to the Golgi region in a fashion similar to PAM. Membrane-tethered PC1 and PC2 exhibited changes in pro-domain maturation rates, N-glycosylation, and in the pH and calcium optima required for maximal enzymatic activity against a fluorogenic substrate. In addition, the PC chimeras efficiently cleaved endogenous pro- opiomelanocortin to the correct bioactive peptides. The PAM transmembrane domain/cytoplasmic domain also prevented stimulated secretion of pro- opiomelanocortin products in AtT-20 cells.

Original languageEnglish (US)
Pages (from-to)24703-24713
Number of pages11
JournalJournal of Biological Chemistry
Volume274
Issue number35
DOIs
StatePublished - Aug 27 1999

Fingerprint

Proprotein Convertase 2
Proprotein Convertase 1
Proprotein Convertases
Pro-Opiomelanocortin
Mixed Function Oxygenases
Chemical activation
Secretory Vesicles
Membranes
Membrane Proteins
Subtilisin
Endocrine Cells
Peptide Hormones
Neuropeptides
Glycosylation
Fluorescent Dyes
Biosynthesis
Neurons
Calcium
Peptides
Cells

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Activation and routing of membrane-tethered prohormone convertases 1 and 2. / Bruzzaniti, Angela; Marx, Ruth; Mains, Richard E.

In: Journal of Biological Chemistry, Vol. 274, No. 35, 27.08.1999, p. 24703-24713.

Research output: Contribution to journalArticle

Bruzzaniti, Angela ; Marx, Ruth ; Mains, Richard E. / Activation and routing of membrane-tethered prohormone convertases 1 and 2. In: Journal of Biological Chemistry. 1999 ; Vol. 274, No. 35. pp. 24703-24713.
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