Activation of AP-1 and increased synthesis of MMP-9 in the rabbit retina induced by lipid hydroperoxide

Shinichi Iwai, Ahmad Aljada, Akira Higa, Takako Nakanishi-Ueda, Shohei Fukuda, Maki Kamegawa, Shigehiro Iwabuchi, Toshihiko Ueda, Sergio Caballero, Richard Browne, Aqeela Afzal, Maria Grant, Hajime Yasuhara, Ryohei Koide, Katsuji Oguchi, Paresh Dandona, Donald Armstrong

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Purpose: We identified the temporal expression of activator protein-1 (AP-1) and matrix metalloproteinases (MMPs) after linoleic acid hydroperoxide (LHP) induction of retinal neovascularization. Methods: After injection of LHP into the vitreous of rabbits, samples were collected for AP-1 binding activity and mRNA for MMP-9 and MMPs activity. AP-1 binding activity was measured by eletrophoretic mobility shift assay. MMP-9 activity was measured by zymography and mRNA by quantitative RT-PCR. Results: AP-1 binding activity was increased at 1-3 hr. MMP-9 mRNA levels were increased at 3 hr in the neural retina and by 12 hr in the retinal pigment epithelium (RPE) layer. MMP-9 proteolytic activity was elevated within the neural retina and within the vitreous and in the RPE-interphotoreceptor matrix (IPM) at 12 hr and peaked at 24 hr or 4 days. Conclusions: LHP increases the transcription factor AP-1 which in turn may regulate retinal MMP-9 synthesis during neovascularization.

Original languageEnglish (US)
Pages (from-to)337-346
Number of pages10
JournalCurrent Eye Research
Volume31
Issue number4
DOIs
StatePublished - May 1 2006
Externally publishedYes

Fingerprint

Lipid Peroxides
Matrix Metalloproteinase 9
Transcription Factor AP-1
Retina
Rabbits
Protein Binding
Retinal Pigment Epithelium
Matrix Metalloproteinases
Messenger RNA
Retinal Neovascularization
Electrophoretic Mobility Shift Assay
Transcription Factors
Polymerase Chain Reaction
Injections
linoleic acid hydroperoxide

Keywords

  • Activator protein-1
  • Lipid hydroperoxide
  • Matrix metalloproteinases
  • Neovascularization
  • Retina

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

Cite this

Iwai, S., Aljada, A., Higa, A., Nakanishi-Ueda, T., Fukuda, S., Kamegawa, M., ... Armstrong, D. (2006). Activation of AP-1 and increased synthesis of MMP-9 in the rabbit retina induced by lipid hydroperoxide. Current Eye Research, 31(4), 337-346. https://doi.org/10.1080/02713680600577780

Activation of AP-1 and increased synthesis of MMP-9 in the rabbit retina induced by lipid hydroperoxide. / Iwai, Shinichi; Aljada, Ahmad; Higa, Akira; Nakanishi-Ueda, Takako; Fukuda, Shohei; Kamegawa, Maki; Iwabuchi, Shigehiro; Ueda, Toshihiko; Caballero, Sergio; Browne, Richard; Afzal, Aqeela; Grant, Maria; Yasuhara, Hajime; Koide, Ryohei; Oguchi, Katsuji; Dandona, Paresh; Armstrong, Donald.

In: Current Eye Research, Vol. 31, No. 4, 01.05.2006, p. 337-346.

Research output: Contribution to journalArticle

Iwai, S, Aljada, A, Higa, A, Nakanishi-Ueda, T, Fukuda, S, Kamegawa, M, Iwabuchi, S, Ueda, T, Caballero, S, Browne, R, Afzal, A, Grant, M, Yasuhara, H, Koide, R, Oguchi, K, Dandona, P & Armstrong, D 2006, 'Activation of AP-1 and increased synthesis of MMP-9 in the rabbit retina induced by lipid hydroperoxide', Current Eye Research, vol. 31, no. 4, pp. 337-346. https://doi.org/10.1080/02713680600577780
Iwai, Shinichi ; Aljada, Ahmad ; Higa, Akira ; Nakanishi-Ueda, Takako ; Fukuda, Shohei ; Kamegawa, Maki ; Iwabuchi, Shigehiro ; Ueda, Toshihiko ; Caballero, Sergio ; Browne, Richard ; Afzal, Aqeela ; Grant, Maria ; Yasuhara, Hajime ; Koide, Ryohei ; Oguchi, Katsuji ; Dandona, Paresh ; Armstrong, Donald. / Activation of AP-1 and increased synthesis of MMP-9 in the rabbit retina induced by lipid hydroperoxide. In: Current Eye Research. 2006 ; Vol. 31, No. 4. pp. 337-346.
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AU - Iwabuchi, Shigehiro

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AU - Dandona, Paresh

AU - Armstrong, Donald

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