Activation of AP-1 and increased synthesis of MMP-9 in the rabbit retina induced by lipid hydroperoxide

Shinichi Iwai, Ahmad Aljada, Akira Higa, Takako Nakanishi-Ueda, Shohei Fukuda, Maki Kamegawa, Shigehiro Iwabuchi, Toshihiko Ueda, Sergio Caballero, Richard Browne, Aqeela Afzal, Maria Grant, Hajime Yasuhara, Ryohei Koide, Katsuji Oguchi, Paresh Dandona, Donald Armstrong

Research output: Contribution to journalArticle

8 Scopus citations


Purpose: We identified the temporal expression of activator protein-1 (AP-1) and matrix metalloproteinases (MMPs) after linoleic acid hydroperoxide (LHP) induction of retinal neovascularization. Methods: After injection of LHP into the vitreous of rabbits, samples were collected for AP-1 binding activity and mRNA for MMP-9 and MMPs activity. AP-1 binding activity was measured by eletrophoretic mobility shift assay. MMP-9 activity was measured by zymography and mRNA by quantitative RT-PCR. Results: AP-1 binding activity was increased at 1-3 hr. MMP-9 mRNA levels were increased at 3 hr in the neural retina and by 12 hr in the retinal pigment epithelium (RPE) layer. MMP-9 proteolytic activity was elevated within the neural retina and within the vitreous and in the RPE-interphotoreceptor matrix (IPM) at 12 hr and peaked at 24 hr or 4 days. Conclusions: LHP increases the transcription factor AP-1 which in turn may regulate retinal MMP-9 synthesis during neovascularization.

Original languageEnglish (US)
Pages (from-to)337-346
Number of pages10
JournalCurrent Eye Research
Issue number4
StatePublished - May 1 2006



  • Activator protein-1
  • Lipid hydroperoxide
  • Matrix metalloproteinases
  • Neovascularization
  • Retina

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Iwai, S., Aljada, A., Higa, A., Nakanishi-Ueda, T., Fukuda, S., Kamegawa, M., Iwabuchi, S., Ueda, T., Caballero, S., Browne, R., Afzal, A., Grant, M., Yasuhara, H., Koide, R., Oguchi, K., Dandona, P., & Armstrong, D. (2006). Activation of AP-1 and increased synthesis of MMP-9 in the rabbit retina induced by lipid hydroperoxide. Current Eye Research, 31(4), 337-346.