Activation of platelet-activating factor receptor in SZ95 sebocytes results in inflammatory cytokine and prostaglandin E2 production

Qiwei Zhang, Holger Seltmann, Christos C. Zouboulis, Jeffrey Travers

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Platelet-activating factor (PAF) is a group of phosphocholines with various biological effects mediated by the PAF receptor (PAF-R). Activation of the epidermal PAF-R induces the expression of inflammatory mediators, including cyclooxygenase-2 (COX-2) and prostaglandin E2 (PGE2). The upregulation of COX-2 expression has been shown to be involved in sebocyte proliferation, sebaceous gland inflammation and carcinogenesis. The present study was designed to investigate whether PAF-R activation could induce the expression of COX-2 and production of PGE2, as well as secretion of the inflammatory cytokine, interleukin-8 (IL-8), in the immortalized sebaceous gland cell line SZ95. Using calcium mobilization studies, we first confirmed that PAF can signal through PAF-R in SZ95 sebocytes. We then found that the production of IL-8 was induced following treatment with PAF-R agonist, however blocked by a specific PAF-R antagonist. Induction of COX-2 expression and increased PGE2 production were observed in SZ95 sebocytes after PAF-R activation. Finally, it was demonstrated that the production of PGE2, induced by PAF-R activation and mediated by COX-2 expression, was blocked following PAF-R antagonism in SZ95 sebocytes. These studies suggest that SZ95 sebocytes express functional PAF-Rs and PAF-Rs are involved in regulating the expression of inflammatory mediators, including COX-2, PGE2 and IL-8.

Original languageEnglish
Pages (from-to)769-774
Number of pages6
JournalExperimental Dermatology
Volume15
Issue number10
DOIs
StatePublished - Oct 2006

Fingerprint

Platelet Activating Factor
Dinoprostone
Chemical activation
Cytokines
Cyclooxygenase 2
Interleukin-8
Sebaceous Glands
platelet activating factor receptor
Phosphorylcholine
Carcinogenesis
Up-Regulation
Cells

Keywords

  • Cyclooxygenase-2
  • Platelet-activating factor
  • Prostaglandin E
  • Sebocytes

ASJC Scopus subject areas

  • Dermatology

Cite this

Activation of platelet-activating factor receptor in SZ95 sebocytes results in inflammatory cytokine and prostaglandin E2 production. / Zhang, Qiwei; Seltmann, Holger; Zouboulis, Christos C.; Travers, Jeffrey.

In: Experimental Dermatology, Vol. 15, No. 10, 10.2006, p. 769-774.

Research output: Contribution to journalArticle

@article{cda51890a43441d38f1a94ac21b38e16,
title = "Activation of platelet-activating factor receptor in SZ95 sebocytes results in inflammatory cytokine and prostaglandin E2 production",
abstract = "Platelet-activating factor (PAF) is a group of phosphocholines with various biological effects mediated by the PAF receptor (PAF-R). Activation of the epidermal PAF-R induces the expression of inflammatory mediators, including cyclooxygenase-2 (COX-2) and prostaglandin E2 (PGE2). The upregulation of COX-2 expression has been shown to be involved in sebocyte proliferation, sebaceous gland inflammation and carcinogenesis. The present study was designed to investigate whether PAF-R activation could induce the expression of COX-2 and production of PGE2, as well as secretion of the inflammatory cytokine, interleukin-8 (IL-8), in the immortalized sebaceous gland cell line SZ95. Using calcium mobilization studies, we first confirmed that PAF can signal through PAF-R in SZ95 sebocytes. We then found that the production of IL-8 was induced following treatment with PAF-R agonist, however blocked by a specific PAF-R antagonist. Induction of COX-2 expression and increased PGE2 production were observed in SZ95 sebocytes after PAF-R activation. Finally, it was demonstrated that the production of PGE2, induced by PAF-R activation and mediated by COX-2 expression, was blocked following PAF-R antagonism in SZ95 sebocytes. These studies suggest that SZ95 sebocytes express functional PAF-Rs and PAF-Rs are involved in regulating the expression of inflammatory mediators, including COX-2, PGE2 and IL-8.",
keywords = "Cyclooxygenase-2, Platelet-activating factor, Prostaglandin E, Sebocytes",
author = "Qiwei Zhang and Holger Seltmann and Zouboulis, {Christos C.} and Jeffrey Travers",
year = "2006",
month = "10",
doi = "10.1111/j.1600-0625.2006.00458.x",
language = "English",
volume = "15",
pages = "769--774",
journal = "Experimental Dermatology",
issn = "0906-6705",
publisher = "Wiley-Blackwell",
number = "10",

}

TY - JOUR

T1 - Activation of platelet-activating factor receptor in SZ95 sebocytes results in inflammatory cytokine and prostaglandin E2 production

AU - Zhang, Qiwei

AU - Seltmann, Holger

AU - Zouboulis, Christos C.

AU - Travers, Jeffrey

PY - 2006/10

Y1 - 2006/10

N2 - Platelet-activating factor (PAF) is a group of phosphocholines with various biological effects mediated by the PAF receptor (PAF-R). Activation of the epidermal PAF-R induces the expression of inflammatory mediators, including cyclooxygenase-2 (COX-2) and prostaglandin E2 (PGE2). The upregulation of COX-2 expression has been shown to be involved in sebocyte proliferation, sebaceous gland inflammation and carcinogenesis. The present study was designed to investigate whether PAF-R activation could induce the expression of COX-2 and production of PGE2, as well as secretion of the inflammatory cytokine, interleukin-8 (IL-8), in the immortalized sebaceous gland cell line SZ95. Using calcium mobilization studies, we first confirmed that PAF can signal through PAF-R in SZ95 sebocytes. We then found that the production of IL-8 was induced following treatment with PAF-R agonist, however blocked by a specific PAF-R antagonist. Induction of COX-2 expression and increased PGE2 production were observed in SZ95 sebocytes after PAF-R activation. Finally, it was demonstrated that the production of PGE2, induced by PAF-R activation and mediated by COX-2 expression, was blocked following PAF-R antagonism in SZ95 sebocytes. These studies suggest that SZ95 sebocytes express functional PAF-Rs and PAF-Rs are involved in regulating the expression of inflammatory mediators, including COX-2, PGE2 and IL-8.

AB - Platelet-activating factor (PAF) is a group of phosphocholines with various biological effects mediated by the PAF receptor (PAF-R). Activation of the epidermal PAF-R induces the expression of inflammatory mediators, including cyclooxygenase-2 (COX-2) and prostaglandin E2 (PGE2). The upregulation of COX-2 expression has been shown to be involved in sebocyte proliferation, sebaceous gland inflammation and carcinogenesis. The present study was designed to investigate whether PAF-R activation could induce the expression of COX-2 and production of PGE2, as well as secretion of the inflammatory cytokine, interleukin-8 (IL-8), in the immortalized sebaceous gland cell line SZ95. Using calcium mobilization studies, we first confirmed that PAF can signal through PAF-R in SZ95 sebocytes. We then found that the production of IL-8 was induced following treatment with PAF-R agonist, however blocked by a specific PAF-R antagonist. Induction of COX-2 expression and increased PGE2 production were observed in SZ95 sebocytes after PAF-R activation. Finally, it was demonstrated that the production of PGE2, induced by PAF-R activation and mediated by COX-2 expression, was blocked following PAF-R antagonism in SZ95 sebocytes. These studies suggest that SZ95 sebocytes express functional PAF-Rs and PAF-Rs are involved in regulating the expression of inflammatory mediators, including COX-2, PGE2 and IL-8.

KW - Cyclooxygenase-2

KW - Platelet-activating factor

KW - Prostaglandin E

KW - Sebocytes

UR - http://www.scopus.com/inward/record.url?scp=33748973412&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33748973412&partnerID=8YFLogxK

U2 - 10.1111/j.1600-0625.2006.00458.x

DO - 10.1111/j.1600-0625.2006.00458.x

M3 - Article

C2 - 16984258

AN - SCOPUS:33748973412

VL - 15

SP - 769

EP - 774

JO - Experimental Dermatology

JF - Experimental Dermatology

SN - 0906-6705

IS - 10

ER -