Activation of platelet-activating factor receptor in SZ95 sebocytes results in inflammatory cytokine and prostaglandin E2 production

Qiwei Zhang, Holger Seltmann, Christos C. Zouboulis, Jeffrey B. Travers

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Abstract

Platelet-activating factor (PAF) is a group of phosphocholines with various biological effects mediated by the PAF receptor (PAF-R). Activation of the epidermal PAF-R induces the expression of inflammatory mediators, including cyclooxygenase-2 (COX-2) and prostaglandin E2 (PGE2). The upregulation of COX-2 expression has been shown to be involved in sebocyte proliferation, sebaceous gland inflammation and carcinogenesis. The present study was designed to investigate whether PAF-R activation could induce the expression of COX-2 and production of PGE2, as well as secretion of the inflammatory cytokine, interleukin-8 (IL-8), in the immortalized sebaceous gland cell line SZ95. Using calcium mobilization studies, we first confirmed that PAF can signal through PAF-R in SZ95 sebocytes. We then found that the production of IL-8 was induced following treatment with PAF-R agonist, however blocked by a specific PAF-R antagonist. Induction of COX-2 expression and increased PGE2 production were observed in SZ95 sebocytes after PAF-R activation. Finally, it was demonstrated that the production of PGE2, induced by PAF-R activation and mediated by COX-2 expression, was blocked following PAF-R antagonism in SZ95 sebocytes. These studies suggest that SZ95 sebocytes express functional PAF-Rs and PAF-Rs are involved in regulating the expression of inflammatory mediators, including COX-2, PGE2 and IL-8.

Original languageEnglish (US)
Pages (from-to)769-774
Number of pages6
JournalExperimental Dermatology
Volume15
Issue number10
DOIs
StatePublished - Oct 1 2006

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Keywords

  • Cyclooxygenase-2
  • Platelet-activating factor
  • Prostaglandin E
  • Sebocytes

ASJC Scopus subject areas

  • Dermatology

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