PURPOSE. To investigate the effect of EGF, IGF-1, and VEGF on ARPE19 cell proliferation and differentiation. METHODS. The gene expression of RPE-specific differentiation and proliferation markers and the transcriptional and translational activity of β-catenin signaling markers were measured by flow cytometry and RT-PCR. RESULTS. The data showed a significant decrease in RPE65, CRALBP, and cytokeratin 18 in ARPE-19 cells stimulated with EGF and IGF-1. In addition, a significant decrease in GSK-3β and β-catenin was observed that was paralleled by an increase in cyclin D1 expression. Cell cycle studies revealed an increase in ARPE cells in the S-G2/M-phase after treatment with EGF or IGF-1. VEGF, on the other hand, led to a reduction in cyclin D1 and to an increase in GSK 3β and β-catenin expression which was paralleled by an increase in RPE-specific differentiation markers. CONCLUSIONS. The data demonstrate the induction of proliferation by EGF and IGF-1 and upregulation of the β-catenin signaling pathway in ARPE-19 cells. The data suggest that activation of the β-catenin signaling pathway may be key in activating ARPE-19 cells by different growth factors.
ASJC Scopus subject areas
- Sensory Systems
- Cellular and Molecular Neuroscience