Activation of the β-catenin signaling pathway and its impact on RPE cell cycle

Kerstin Steindl-Kuscher, Walter Krugluger, Michael E. Boulton, Paulina Haas, Karl Schrattbauer, Hans Feichtinger, Wolfram Adlassnig, Susanne Binder

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17 Scopus citations

Abstract

PURPOSE. To investigate the effect of EGF, IGF-1, and VEGF on ARPE19 cell proliferation and differentiation. METHODS. The gene expression of RPE-specific differentiation and proliferation markers and the transcriptional and translational activity of β-catenin signaling markers were measured by flow cytometry and RT-PCR. RESULTS. The data showed a significant decrease in RPE65, CRALBP, and cytokeratin 18 in ARPE-19 cells stimulated with EGF and IGF-1. In addition, a significant decrease in GSK-3β and β-catenin was observed that was paralleled by an increase in cyclin D1 expression. Cell cycle studies revealed an increase in ARPE cells in the S-G2/M-phase after treatment with EGF or IGF-1. VEGF, on the other hand, led to a reduction in cyclin D1 and to an increase in GSK 3β and β-catenin expression which was paralleled by an increase in RPE-specific differentiation markers. CONCLUSIONS. The data demonstrate the induction of proliferation by EGF and IGF-1 and upregulation of the β-catenin signaling pathway in ARPE-19 cells. The data suggest that activation of the β-catenin signaling pathway may be key in activating ARPE-19 cells by different growth factors.

Original languageEnglish (US)
Pages (from-to)4471-4476
Number of pages6
JournalInvestigative Ophthalmology and Visual Science
Volume50
Issue number9
DOIs
StatePublished - Dec 1 2009

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ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Steindl-Kuscher, K., Krugluger, W., Boulton, M. E., Haas, P., Schrattbauer, K., Feichtinger, H., Adlassnig, W., & Binder, S. (2009). Activation of the β-catenin signaling pathway and its impact on RPE cell cycle. Investigative Ophthalmology and Visual Science, 50(9), 4471-4476. https://doi.org/10.1167/iovs.08-3139