Activation of the β-catenin signaling pathway and its impact on RPE cell cycle

Kerstin Steindl-Kuscher, Walter Krugluger, Michael E. Boulton, Paulina Haas, Karl Schrattbauer, Hans Feichtinger, Wolfram Adlassnig, Susanne Binder

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

PURPOSE. To investigate the effect of EGF, IGF-1, and VEGF on ARPE19 cell proliferation and differentiation. METHODS. The gene expression of RPE-specific differentiation and proliferation markers and the transcriptional and translational activity of β-catenin signaling markers were measured by flow cytometry and RT-PCR. RESULTS. The data showed a significant decrease in RPE65, CRALBP, and cytokeratin 18 in ARPE-19 cells stimulated with EGF and IGF-1. In addition, a significant decrease in GSK-3β and β-catenin was observed that was paralleled by an increase in cyclin D1 expression. Cell cycle studies revealed an increase in ARPE cells in the S-G2/M-phase after treatment with EGF or IGF-1. VEGF, on the other hand, led to a reduction in cyclin D1 and to an increase in GSK 3β and β-catenin expression which was paralleled by an increase in RPE-specific differentiation markers. CONCLUSIONS. The data demonstrate the induction of proliferation by EGF and IGF-1 and upregulation of the β-catenin signaling pathway in ARPE-19 cells. The data suggest that activation of the β-catenin signaling pathway may be key in activating ARPE-19 cells by different growth factors.

Original languageEnglish (US)
Pages (from-to)4471-4476
Number of pages6
JournalInvestigative Ophthalmology and Visual Science
Volume50
Issue number9
DOIs
StatePublished - 2009
Externally publishedYes

Fingerprint

Catenins
Cell Cycle
Insulin-Like Growth Factor I
Epidermal Growth Factor
Glycogen Synthase Kinase 3
Cyclin D1
Differentiation Antigens
Vascular Endothelial Growth Factor A
Keratin-18
G2 Phase
Cell Division
Cell Differentiation
Intercellular Signaling Peptides and Proteins
Flow Cytometry
Up-Regulation
Cell Proliferation
Gene Expression
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience
  • Medicine(all)

Cite this

Steindl-Kuscher, K., Krugluger, W., Boulton, M. E., Haas, P., Schrattbauer, K., Feichtinger, H., ... Binder, S. (2009). Activation of the β-catenin signaling pathway and its impact on RPE cell cycle. Investigative Ophthalmology and Visual Science, 50(9), 4471-4476. https://doi.org/10.1167/iovs.08-3139

Activation of the β-catenin signaling pathway and its impact on RPE cell cycle. / Steindl-Kuscher, Kerstin; Krugluger, Walter; Boulton, Michael E.; Haas, Paulina; Schrattbauer, Karl; Feichtinger, Hans; Adlassnig, Wolfram; Binder, Susanne.

In: Investigative Ophthalmology and Visual Science, Vol. 50, No. 9, 2009, p. 4471-4476.

Research output: Contribution to journalArticle

Steindl-Kuscher, K, Krugluger, W, Boulton, ME, Haas, P, Schrattbauer, K, Feichtinger, H, Adlassnig, W & Binder, S 2009, 'Activation of the β-catenin signaling pathway and its impact on RPE cell cycle', Investigative Ophthalmology and Visual Science, vol. 50, no. 9, pp. 4471-4476. https://doi.org/10.1167/iovs.08-3139
Steindl-Kuscher K, Krugluger W, Boulton ME, Haas P, Schrattbauer K, Feichtinger H et al. Activation of the β-catenin signaling pathway and its impact on RPE cell cycle. Investigative Ophthalmology and Visual Science. 2009;50(9):4471-4476. https://doi.org/10.1167/iovs.08-3139
Steindl-Kuscher, Kerstin ; Krugluger, Walter ; Boulton, Michael E. ; Haas, Paulina ; Schrattbauer, Karl ; Feichtinger, Hans ; Adlassnig, Wolfram ; Binder, Susanne. / Activation of the β-catenin signaling pathway and its impact on RPE cell cycle. In: Investigative Ophthalmology and Visual Science. 2009 ; Vol. 50, No. 9. pp. 4471-4476.
@article{9b158a73ac574b919b1c56c5e2a6f220,
title = "Activation of the β-catenin signaling pathway and its impact on RPE cell cycle",
abstract = "PURPOSE. To investigate the effect of EGF, IGF-1, and VEGF on ARPE19 cell proliferation and differentiation. METHODS. The gene expression of RPE-specific differentiation and proliferation markers and the transcriptional and translational activity of β-catenin signaling markers were measured by flow cytometry and RT-PCR. RESULTS. The data showed a significant decrease in RPE65, CRALBP, and cytokeratin 18 in ARPE-19 cells stimulated with EGF and IGF-1. In addition, a significant decrease in GSK-3β and β-catenin was observed that was paralleled by an increase in cyclin D1 expression. Cell cycle studies revealed an increase in ARPE cells in the S-G2/M-phase after treatment with EGF or IGF-1. VEGF, on the other hand, led to a reduction in cyclin D1 and to an increase in GSK 3β and β-catenin expression which was paralleled by an increase in RPE-specific differentiation markers. CONCLUSIONS. The data demonstrate the induction of proliferation by EGF and IGF-1 and upregulation of the β-catenin signaling pathway in ARPE-19 cells. The data suggest that activation of the β-catenin signaling pathway may be key in activating ARPE-19 cells by different growth factors.",
author = "Kerstin Steindl-Kuscher and Walter Krugluger and Boulton, {Michael E.} and Paulina Haas and Karl Schrattbauer and Hans Feichtinger and Wolfram Adlassnig and Susanne Binder",
year = "2009",
doi = "10.1167/iovs.08-3139",
language = "English (US)",
volume = "50",
pages = "4471--4476",
journal = "Investigative Ophthalmology and Visual Science",
issn = "0146-0404",
publisher = "Association for Research in Vision and Ophthalmology Inc.",
number = "9",

}

TY - JOUR

T1 - Activation of the β-catenin signaling pathway and its impact on RPE cell cycle

AU - Steindl-Kuscher, Kerstin

AU - Krugluger, Walter

AU - Boulton, Michael E.

AU - Haas, Paulina

AU - Schrattbauer, Karl

AU - Feichtinger, Hans

AU - Adlassnig, Wolfram

AU - Binder, Susanne

PY - 2009

Y1 - 2009

N2 - PURPOSE. To investigate the effect of EGF, IGF-1, and VEGF on ARPE19 cell proliferation and differentiation. METHODS. The gene expression of RPE-specific differentiation and proliferation markers and the transcriptional and translational activity of β-catenin signaling markers were measured by flow cytometry and RT-PCR. RESULTS. The data showed a significant decrease in RPE65, CRALBP, and cytokeratin 18 in ARPE-19 cells stimulated with EGF and IGF-1. In addition, a significant decrease in GSK-3β and β-catenin was observed that was paralleled by an increase in cyclin D1 expression. Cell cycle studies revealed an increase in ARPE cells in the S-G2/M-phase after treatment with EGF or IGF-1. VEGF, on the other hand, led to a reduction in cyclin D1 and to an increase in GSK 3β and β-catenin expression which was paralleled by an increase in RPE-specific differentiation markers. CONCLUSIONS. The data demonstrate the induction of proliferation by EGF and IGF-1 and upregulation of the β-catenin signaling pathway in ARPE-19 cells. The data suggest that activation of the β-catenin signaling pathway may be key in activating ARPE-19 cells by different growth factors.

AB - PURPOSE. To investigate the effect of EGF, IGF-1, and VEGF on ARPE19 cell proliferation and differentiation. METHODS. The gene expression of RPE-specific differentiation and proliferation markers and the transcriptional and translational activity of β-catenin signaling markers were measured by flow cytometry and RT-PCR. RESULTS. The data showed a significant decrease in RPE65, CRALBP, and cytokeratin 18 in ARPE-19 cells stimulated with EGF and IGF-1. In addition, a significant decrease in GSK-3β and β-catenin was observed that was paralleled by an increase in cyclin D1 expression. Cell cycle studies revealed an increase in ARPE cells in the S-G2/M-phase after treatment with EGF or IGF-1. VEGF, on the other hand, led to a reduction in cyclin D1 and to an increase in GSK 3β and β-catenin expression which was paralleled by an increase in RPE-specific differentiation markers. CONCLUSIONS. The data demonstrate the induction of proliferation by EGF and IGF-1 and upregulation of the β-catenin signaling pathway in ARPE-19 cells. The data suggest that activation of the β-catenin signaling pathway may be key in activating ARPE-19 cells by different growth factors.

UR - http://www.scopus.com/inward/record.url?scp=70349233977&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70349233977&partnerID=8YFLogxK

U2 - 10.1167/iovs.08-3139

DO - 10.1167/iovs.08-3139

M3 - Article

VL - 50

SP - 4471

EP - 4476

JO - Investigative Ophthalmology and Visual Science

JF - Investigative Ophthalmology and Visual Science

SN - 0146-0404

IS - 9

ER -