Activation of the wnt pathway plays a pathogenic role in diabetic retinopathy in humans and animal models

Ying Chen, Yang Hu, Ti Zhou, Kevin K. Zhou, Robert Mott, Mingyuan Wu, Michael Boulton, Timothy J. Lyons, Guoquan Gao, Jian Xing Ma

Research output: Contribution to journalArticle

102 Scopus citations

Abstract

Although Wnt signaling is known to mediate multiple biological and pathological processes, its association with diabetic retinopathy (DR) has not been established. Here we show that retinal levels and nuclear translocation of β-catenin, a key effector in the canonical Wnt pathway, were increased in humans with DR and in three DR models. Retinal levels of lowdensity lipoprotein receptor-related proteins 5 and 6, coreceptors of Wnts, were also elevated in the DR models. The high glucose-induced activation of β-catenin was attenuated by aminoguanidine, suggesting that oxidative stress is a direct cause for the Wnt pathway activation in diabetes. Indeed, Dickkopf homolog 1, a specific inhibitor of the Wnt pathway, ameliorated retinal inflammation, vascular leakage, and retinal neovascularization in the DR models. Dickkopf homolog 1 also blocked the generation of reactive oxygen species induced by high glucose, suggesting that Wnt signaling contributes to the oxidative stress in diabetes. These observations indicate that the Wnt pathway plays a pathogenic role in DR and represents a novel therapeutic target.

Original languageEnglish (US)
Pages (from-to)2676-2685
Number of pages10
JournalAmerican Journal of Pathology
Volume175
Issue number6
DOIs
StatePublished - 2009

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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    Chen, Y., Hu, Y., Zhou, T., Zhou, K. K., Mott, R., Wu, M., Boulton, M., Lyons, T. J., Gao, G., & Ma, J. X. (2009). Activation of the wnt pathway plays a pathogenic role in diabetic retinopathy in humans and animal models. American Journal of Pathology, 175(6), 2676-2685. https://doi.org/10.2353/ajpath.2009.080945