Activation of Vascular Endothelial Growth Factor Receptor-1 Sustains Angiogenesis and Bcl-2 Expression Via the Phosphatidylinositol 3-Kinase Pathway in Endothelial Cells

Jun Cai, Shakil Ahmad, Wen G. Jiang, Jianhua Huang, Christopher D. Kontos, Mike Boulton, Asif Ahmed

Research output: Contribution to journalArticle

112 Citations (Scopus)

Abstract

Vascular insufficiency and retinal ischemia precede many proliferative retinopathies and stimulate secretion of various vasoactive growth factors, including vascular endothelial growth factor (VEGF) and placenta growth factor (PlGF). It is unclear, however, how PlGF, which is elevated in proliferative diabetic retinopathy and is a VEGF homolog that binds only to VEGF receptor (VEGFR)-1, promotes pathological angiogenesis. When primary microvascular endothelial cells were grown on collagen gels, PlGF-containing ligands upregulated Bcl-2 expression and stimulated the formation of capillary-like tube networks that were retained for up to 14 days in culture. The inhibition of VEGFR-1 results in a dramatic decrease in the number of capillary connections, indicating that VEGFR-1 ligands promote branching angiogenesis. In contrast, VEGF-induced tube formations and Bcl-2 expression were significantly decreased at the end of this period. Flow cytometry analysis of annexin-V/propidium iodide-stained cells revealed that PlGF and PlGF/VEGF heterodimer inhibited apoptosis in serum-deprived endothelial cells. These two growth factors stimulated a survival signaling pathway phosphatidylinositol 3-kinase (PI3K), as identified by increased Akt phosphorylation and because blocking PI3K signalling by adenovirus-mediated overexpression of wild-type phosphatase and tensin homolog on chromosome 10 (PTEN) disrupted angiogenesis and decreased Bcl-2 expression by PlGF and PlGF/VEGF heterodimer, whereas a dominant-negative PTEN mutant enhanced endothelial sprout formation and Bcl-2 expression. Together, these findings indicate that PlGF-containing ligands contribute to pathological angiogenesis by prolonging cell survival signals and maintaining vascular networks.

Original languageEnglish (US)
Pages (from-to)2959-2968
Number of pages10
JournalDiabetes
Volume52
Issue number12
DOIs
StatePublished - Dec 2003
Externally publishedYes

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Phosphatidylinositol 3-Kinase
Vascular Endothelial Growth Factor Receptor-1
Endothelial Cells
Vascular Endothelial Growth Factor A
Pathologic Neovascularization
Vascular Endothelial Growth Factor Receptor
Ligands
Blood Vessels
Intercellular Signaling Peptides and Proteins
Chromosomes, Human, Pair 10
Placenta Growth Factor
Propidium
Annexin A5
Diabetic Retinopathy
Phosphoric Monoester Hydrolases
Adenoviridae
Cell Survival
Flow Cytometry
Collagen
Ischemia

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Activation of Vascular Endothelial Growth Factor Receptor-1 Sustains Angiogenesis and Bcl-2 Expression Via the Phosphatidylinositol 3-Kinase Pathway in Endothelial Cells. / Cai, Jun; Ahmad, Shakil; Jiang, Wen G.; Huang, Jianhua; Kontos, Christopher D.; Boulton, Mike; Ahmed, Asif.

In: Diabetes, Vol. 52, No. 12, 12.2003, p. 2959-2968.

Research output: Contribution to journalArticle

Cai, Jun ; Ahmad, Shakil ; Jiang, Wen G. ; Huang, Jianhua ; Kontos, Christopher D. ; Boulton, Mike ; Ahmed, Asif. / Activation of Vascular Endothelial Growth Factor Receptor-1 Sustains Angiogenesis and Bcl-2 Expression Via the Phosphatidylinositol 3-Kinase Pathway in Endothelial Cells. In: Diabetes. 2003 ; Vol. 52, No. 12. pp. 2959-2968.
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