Active PSF shaping and adaptive optics enable volumetric localization microscopy through brain sections

Michael J. Mlodzianoski, Paul J. Cheng-Hathaway, Shane M. Bemiller, Tyler J. McCray, Sheng Liu, David A. Miller, Bruce Lamb, Gary E. Landreth, Fang Huang

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Application of single-molecule switching nanoscopy (SMSN) beyond the coverslip surface poses substantial challenges due to sample-induced aberrations that distort and blur single-molecule emission patterns. We combined active shaping of point spread functions and efficient adaptive optics to enable robust 3D-SMSN imaging within tissues. This development allowed us to image through 30-μm-thick brain sections to visualize and reconstruct the morphology and the nanoscale details of amyloid-β filaments in a mouse model of Alzheimer’s disease.

Original languageEnglish (US)
Pages (from-to)1-4
Number of pages4
JournalNature Methods
DOIs
StateAccepted/In press - Jul 16 2018

Fingerprint

Adaptive optics
Amyloid
Microscopy
Brain
Microscopic examination
Alzheimer Disease
Molecules
Optical transfer function
Aberrations
Tissue
Imaging techniques

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Mlodzianoski, M. J., Cheng-Hathaway, P. J., Bemiller, S. M., McCray, T. J., Liu, S., Miller, D. A., ... Huang, F. (Accepted/In press). Active PSF shaping and adaptive optics enable volumetric localization microscopy through brain sections. Nature Methods, 1-4. https://doi.org/10.1038/s41592-018-0053-8

Active PSF shaping and adaptive optics enable volumetric localization microscopy through brain sections. / Mlodzianoski, Michael J.; Cheng-Hathaway, Paul J.; Bemiller, Shane M.; McCray, Tyler J.; Liu, Sheng; Miller, David A.; Lamb, Bruce; Landreth, Gary E.; Huang, Fang.

In: Nature Methods, 16.07.2018, p. 1-4.

Research output: Contribution to journalArticle

Mlodzianoski, MJ, Cheng-Hathaway, PJ, Bemiller, SM, McCray, TJ, Liu, S, Miller, DA, Lamb, B, Landreth, GE & Huang, F 2018, 'Active PSF shaping and adaptive optics enable volumetric localization microscopy through brain sections', Nature Methods, pp. 1-4. https://doi.org/10.1038/s41592-018-0053-8
Mlodzianoski, Michael J. ; Cheng-Hathaway, Paul J. ; Bemiller, Shane M. ; McCray, Tyler J. ; Liu, Sheng ; Miller, David A. ; Lamb, Bruce ; Landreth, Gary E. ; Huang, Fang. / Active PSF shaping and adaptive optics enable volumetric localization microscopy through brain sections. In: Nature Methods. 2018 ; pp. 1-4.
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