Active PSF shaping and adaptive optics enable volumetric localization microscopy through brain sections

Michael J. Mlodzianoski, Paul J. Cheng-Hathaway, Shane M. Bemiller, Tyler J. McCray, Sheng Liu, David A. Miller, Bruce T. Lamb, Gary E. Landreth, Fang Huang

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

Application of single-molecule switching nanoscopy (SMSN) beyond the coverslip surface poses substantial challenges due to sample-induced aberrations that distort and blur single-molecule emission patterns. We combined active shaping of point spread functions and efficient adaptive optics to enable robust 3D-SMSN imaging within tissues. This development allowed us to image through 30-μm-thick brain sections to visualize and reconstruct the morphology and the nanoscale details of amyloid-β filaments in a mouse model of Alzheimer’s disease.

Original languageEnglish (US)
Pages (from-to)583-586
Number of pages4
JournalNature Methods
Volume15
Issue number8
DOIs
StatePublished - Aug 1 2018

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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