Activity and enhancer binding factors for jc virus regulatory elements in differentiating embryonal carcinoma cells

Harikrishna Nakshatri, Alan Pater, Mary M. Pater

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We have studied cell-type-specific expression by JC virus (JCV) DNA regulatory sequences using embryonal carcinoma (EC) cells as a model system. In transient transfection assays, JCV enhancer demonstrated activity in retinoic acid-differentiated neuronal type cells but not in undifferentiated or DMSO-differentiated muscle type cells. To correlate in vivo activity with the binding of transcription factors, we performed DNasel footprinting experiments. Retinoic acidtreated EC cell extracts provided three completely protected regions, each containing sequences with homology to nuclear factor 1 (NF1) binding motifs and the partially protected TATA box. Oligonucleotide competition studies suggest that all three NF1 binding motifs are bound by the same factors but with different affinities and that there are cooperative interactions between NF1 proteins binding to adjacent regions. No protected region other than the partially protected TATA box was detected in undifferentiated and DMSO-differentiated EC cells in which JC regulatory sequences were not expressed.

Original languageEnglish (US)
Pages (from-to)784-789
Number of pages6
JournalVirology
Volume177
Issue number2
DOIs
StatePublished - 1990
Externally publishedYes

Fingerprint

NFI Transcription Factors
Embryonal Carcinoma Stem Cells
JC Virus
TATA Box
Dimethyl Sulfoxide
Viruses
Sequence Homology
Tretinoin
Cell Extracts
Protein Binding
Oligonucleotides
Muscle Cells
Transfection
Transcription Factors

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Activity and enhancer binding factors for jc virus regulatory elements in differentiating embryonal carcinoma cells. / Nakshatri, Harikrishna; Pater, Alan; Pater, Mary M.

In: Virology, Vol. 177, No. 2, 1990, p. 784-789.

Research output: Contribution to journalArticle

@article{66372f98fdfd4ab6b69f4872af781227,
title = "Activity and enhancer binding factors for jc virus regulatory elements in differentiating embryonal carcinoma cells",
abstract = "We have studied cell-type-specific expression by JC virus (JCV) DNA regulatory sequences using embryonal carcinoma (EC) cells as a model system. In transient transfection assays, JCV enhancer demonstrated activity in retinoic acid-differentiated neuronal type cells but not in undifferentiated or DMSO-differentiated muscle type cells. To correlate in vivo activity with the binding of transcription factors, we performed DNasel footprinting experiments. Retinoic acidtreated EC cell extracts provided three completely protected regions, each containing sequences with homology to nuclear factor 1 (NF1) binding motifs and the partially protected TATA box. Oligonucleotide competition studies suggest that all three NF1 binding motifs are bound by the same factors but with different affinities and that there are cooperative interactions between NF1 proteins binding to adjacent regions. No protected region other than the partially protected TATA box was detected in undifferentiated and DMSO-differentiated EC cells in which JC regulatory sequences were not expressed.",
author = "Harikrishna Nakshatri and Alan Pater and Pater, {Mary M.}",
year = "1990",
doi = "10.1016/0042-6822(90)90550-B",
language = "English (US)",
volume = "177",
pages = "784--789",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Activity and enhancer binding factors for jc virus regulatory elements in differentiating embryonal carcinoma cells

AU - Nakshatri, Harikrishna

AU - Pater, Alan

AU - Pater, Mary M.

PY - 1990

Y1 - 1990

N2 - We have studied cell-type-specific expression by JC virus (JCV) DNA regulatory sequences using embryonal carcinoma (EC) cells as a model system. In transient transfection assays, JCV enhancer demonstrated activity in retinoic acid-differentiated neuronal type cells but not in undifferentiated or DMSO-differentiated muscle type cells. To correlate in vivo activity with the binding of transcription factors, we performed DNasel footprinting experiments. Retinoic acidtreated EC cell extracts provided three completely protected regions, each containing sequences with homology to nuclear factor 1 (NF1) binding motifs and the partially protected TATA box. Oligonucleotide competition studies suggest that all three NF1 binding motifs are bound by the same factors but with different affinities and that there are cooperative interactions between NF1 proteins binding to adjacent regions. No protected region other than the partially protected TATA box was detected in undifferentiated and DMSO-differentiated EC cells in which JC regulatory sequences were not expressed.

AB - We have studied cell-type-specific expression by JC virus (JCV) DNA regulatory sequences using embryonal carcinoma (EC) cells as a model system. In transient transfection assays, JCV enhancer demonstrated activity in retinoic acid-differentiated neuronal type cells but not in undifferentiated or DMSO-differentiated muscle type cells. To correlate in vivo activity with the binding of transcription factors, we performed DNasel footprinting experiments. Retinoic acidtreated EC cell extracts provided three completely protected regions, each containing sequences with homology to nuclear factor 1 (NF1) binding motifs and the partially protected TATA box. Oligonucleotide competition studies suggest that all three NF1 binding motifs are bound by the same factors but with different affinities and that there are cooperative interactions between NF1 proteins binding to adjacent regions. No protected region other than the partially protected TATA box was detected in undifferentiated and DMSO-differentiated EC cells in which JC regulatory sequences were not expressed.

UR - http://www.scopus.com/inward/record.url?scp=0025327556&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025327556&partnerID=8YFLogxK

U2 - 10.1016/0042-6822(90)90550-B

DO - 10.1016/0042-6822(90)90550-B

M3 - Article

C2 - 2164735

AN - SCOPUS:0025327556

VL - 177

SP - 784

EP - 789

JO - Virology

JF - Virology

SN - 0042-6822

IS - 2

ER -