Activity-based probes for protein tyrosine phosphatases

Sanjai Kumar, Bo Zhou, Fubo Liang, Wei Qing Wang, Zhonghui Huang, Zhong-Yin Zhang

Research output: Contribution to journalArticle

131 Citations (Scopus)

Abstract

Protein tyrosine phosphatases (PTPs) are involved in the regulation of many aspects of cellular activity including proliferation, differentiation, metabolism, migration, and survival. Given the large number and complexity of PTPs in cell signaling, new strategies are needed for the integrated analysis of PTPs in the whole proteome. Unfortunately, the activities of many PTPs are tightly regulated by posttranslational mechanisms, limiting the utility of standard genomics and proteomics methods for functional characterization of these enzymes. To facilitate the global analysis of PTPs, we designed and synthesized two activity-based probes that consist of α -bromobenzylphosphonate as a PTP-specific trapping device and a linker that connects the trapping device with a biotin tag for visualization and purification. We showed that these probes are active site-directed irreversible inactivators of PTPs and form a covalent adduct with PTPs involving the active site Cys residue. Additionally, we demonstrated that the probes are extremely specific toward PTPs while remaining inert to other proteins, including the whole proteome from Escherichia coli. Consequently, these activity-based PTP probes can be used to profile PTP activity in complex proteomes. The ability to interrogate the entire PTP family on the basis of changes in their activity should greatly accelerate both the assignment of PTP function and the identification of potential therapeutic targets.

Original languageEnglish (US)
Pages (from-to)7943-7948
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume101
Issue number21
DOIs
StatePublished - May 21 2004
Externally publishedYes

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Protein Tyrosine Phosphatases
Proteome
Catalytic Domain
Equipment and Supplies
Biotin
Genomics
Proteomics

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Activity-based probes for protein tyrosine phosphatases. / Kumar, Sanjai; Zhou, Bo; Liang, Fubo; Wang, Wei Qing; Huang, Zhonghui; Zhang, Zhong-Yin.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, No. 21, 21.05.2004, p. 7943-7948.

Research output: Contribution to journalArticle

Kumar, Sanjai ; Zhou, Bo ; Liang, Fubo ; Wang, Wei Qing ; Huang, Zhonghui ; Zhang, Zhong-Yin. / Activity-based probes for protein tyrosine phosphatases. In: Proceedings of the National Academy of Sciences of the United States of America. 2004 ; Vol. 101, No. 21. pp. 7943-7948.
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