Activity of topoisomerase inhibitors against Pneumocystis carinii in vitro and in an inoculated mouse model

J. A. Fishman, Sherry Queener, R. S. Roth, M. S. Bartlett

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Five topoisomerase II inhibitors (amsacrine [m-AMSA], two epipodophyllotoxins, and two quinolones) and the alkaloid camptothecin (a topoisomerase I inhibitor) were evaluated to assess their activities against Pneumocystis carinii. In vitro, both etoposide (VP-16) and teniposide (VM- 26) at 1 μg/ml suppressed P. carinii growth. Amsacrine was toxic to P. carinii and to the feeder cells in vitro. Camptothecin suppressed the growth of P. carinii in vitro only at 100 μg/ml. Studies in immunosuppressed mice demonstrated the efficacy of teniposide against P. carinii pneumonia, but successful administration of teniposide was schedule dependent with significant toxicity at therapeutic dosages.

Original languageEnglish (US)
Pages (from-to)1543-1546
Number of pages4
JournalAntimicrobial Agents and Chemotherapy
Volume37
Issue number7
StatePublished - 1993
Externally publishedYes

Fingerprint

Topoisomerase Inhibitors
Teniposide
Pneumocystis carinii
Amsacrine
Camptothecin
Etoposide
Podophyllotoxin
Topoisomerase I Inhibitors
Topoisomerase II Inhibitors
Feeder Cells
Pneumocystis Pneumonia
Poisons
Quinolones
Growth
Alkaloids
Appointments and Schedules
In Vitro Techniques

ASJC Scopus subject areas

  • Pharmacology (medical)

Cite this

Activity of topoisomerase inhibitors against Pneumocystis carinii in vitro and in an inoculated mouse model. / Fishman, J. A.; Queener, Sherry; Roth, R. S.; Bartlett, M. S.

In: Antimicrobial Agents and Chemotherapy, Vol. 37, No. 7, 1993, p. 1543-1546.

Research output: Contribution to journalArticle

@article{9d1896537cc54c7b88682525b3681097,
title = "Activity of topoisomerase inhibitors against Pneumocystis carinii in vitro and in an inoculated mouse model",
abstract = "Five topoisomerase II inhibitors (amsacrine [m-AMSA], two epipodophyllotoxins, and two quinolones) and the alkaloid camptothecin (a topoisomerase I inhibitor) were evaluated to assess their activities against Pneumocystis carinii. In vitro, both etoposide (VP-16) and teniposide (VM- 26) at 1 μg/ml suppressed P. carinii growth. Amsacrine was toxic to P. carinii and to the feeder cells in vitro. Camptothecin suppressed the growth of P. carinii in vitro only at 100 μg/ml. Studies in immunosuppressed mice demonstrated the efficacy of teniposide against P. carinii pneumonia, but successful administration of teniposide was schedule dependent with significant toxicity at therapeutic dosages.",
author = "Fishman, {J. A.} and Sherry Queener and Roth, {R. S.} and Bartlett, {M. S.}",
year = "1993",
language = "English (US)",
volume = "37",
pages = "1543--1546",
journal = "Antimicrobial Agents and Chemotherapy",
issn = "0066-4804",
publisher = "American Society for Microbiology",
number = "7",

}

TY - JOUR

T1 - Activity of topoisomerase inhibitors against Pneumocystis carinii in vitro and in an inoculated mouse model

AU - Fishman, J. A.

AU - Queener, Sherry

AU - Roth, R. S.

AU - Bartlett, M. S.

PY - 1993

Y1 - 1993

N2 - Five topoisomerase II inhibitors (amsacrine [m-AMSA], two epipodophyllotoxins, and two quinolones) and the alkaloid camptothecin (a topoisomerase I inhibitor) were evaluated to assess their activities against Pneumocystis carinii. In vitro, both etoposide (VP-16) and teniposide (VM- 26) at 1 μg/ml suppressed P. carinii growth. Amsacrine was toxic to P. carinii and to the feeder cells in vitro. Camptothecin suppressed the growth of P. carinii in vitro only at 100 μg/ml. Studies in immunosuppressed mice demonstrated the efficacy of teniposide against P. carinii pneumonia, but successful administration of teniposide was schedule dependent with significant toxicity at therapeutic dosages.

AB - Five topoisomerase II inhibitors (amsacrine [m-AMSA], two epipodophyllotoxins, and two quinolones) and the alkaloid camptothecin (a topoisomerase I inhibitor) were evaluated to assess their activities against Pneumocystis carinii. In vitro, both etoposide (VP-16) and teniposide (VM- 26) at 1 μg/ml suppressed P. carinii growth. Amsacrine was toxic to P. carinii and to the feeder cells in vitro. Camptothecin suppressed the growth of P. carinii in vitro only at 100 μg/ml. Studies in immunosuppressed mice demonstrated the efficacy of teniposide against P. carinii pneumonia, but successful administration of teniposide was schedule dependent with significant toxicity at therapeutic dosages.

UR - http://www.scopus.com/inward/record.url?scp=0027235455&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027235455&partnerID=8YFLogxK

M3 - Article

C2 - 8395791

AN - SCOPUS:0027235455

VL - 37

SP - 1543

EP - 1546

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

SN - 0066-4804

IS - 7

ER -