Acute and chronic effect of insulin on leptin production in humans

Studies in vivo and in vitro

Jerzy W. Kolaczynski, Mark R. Nyce, Robert Considine, Guenther Boden, John J. Nolan, Robert Henry, Sunder R. Mudaliar, Jerrold Olefsky, Jose F. Caro

Research output: Contribution to journalArticle

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Abstract

This study was undertaken to investigate the changes in obesity (OB) gene expression and production of leptin in response to insulin in vitro and in vivo under euglycemic and hyperglycemic conditions in humans. Three protocols were used: 1) euglycemic clump with insulin infusion rates at 40, 120, 300, and 1,200 mU · m-2 · min-1 carried out for up to 5 h performed in 16 normal lean individuals, 30 obese individuals, and 31 patients with NIDDM; 2) 64-to 72-h hyperglycemic (glucose 12.6 mmol/l) clamp performed on 5 lean individuals; 3) long-term (96-h) primary culture of isolated abdominal adipocytes in the presence and absence of 100 nmol/l insulin. Short-term hyperinsulinemia in the range of 80 to > 10,000 μU/ml had no effect on circulating levels of leptin. During the prolonged hyperglycemic clamp, a rise in leptin was observed during the last 24 h of the study (P <0.001). In the presence of insulin in vitro, OB gene expression increased at 72 h (P <0.01), followed by an increase in leptin released to the medium (P <0.001). In summary, insulin does not stimulate leptin production acutely; however, a long-term effect of insulin on leptin production could be demonstrated both in vivo and in vitro. These data suggest that insulin regulates OB gene expression and leptin production indirectly, probably through its trophic effect on adipocytes.

Original languageEnglish (US)
Pages (from-to)699-701
Number of pages3
JournalDiabetes
Volume45
Issue number5
StatePublished - May 1996
Externally publishedYes

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Leptin
Insulin
Obesity
Gene Expression
Adipocytes
Hyperinsulinism
In Vitro Techniques
Type 2 Diabetes Mellitus
Glucose

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Internal Medicine

Cite this

Kolaczynski, J. W., Nyce, M. R., Considine, R., Boden, G., Nolan, J. J., Henry, R., ... Caro, J. F. (1996). Acute and chronic effect of insulin on leptin production in humans: Studies in vivo and in vitro. Diabetes, 45(5), 699-701.

Acute and chronic effect of insulin on leptin production in humans : Studies in vivo and in vitro. / Kolaczynski, Jerzy W.; Nyce, Mark R.; Considine, Robert; Boden, Guenther; Nolan, John J.; Henry, Robert; Mudaliar, Sunder R.; Olefsky, Jerrold; Caro, Jose F.

In: Diabetes, Vol. 45, No. 5, 05.1996, p. 699-701.

Research output: Contribution to journalArticle

Kolaczynski, JW, Nyce, MR, Considine, R, Boden, G, Nolan, JJ, Henry, R, Mudaliar, SR, Olefsky, J & Caro, JF 1996, 'Acute and chronic effect of insulin on leptin production in humans: Studies in vivo and in vitro', Diabetes, vol. 45, no. 5, pp. 699-701.
Kolaczynski JW, Nyce MR, Considine R, Boden G, Nolan JJ, Henry R et al. Acute and chronic effect of insulin on leptin production in humans: Studies in vivo and in vitro. Diabetes. 1996 May;45(5):699-701.
Kolaczynski, Jerzy W. ; Nyce, Mark R. ; Considine, Robert ; Boden, Guenther ; Nolan, John J. ; Henry, Robert ; Mudaliar, Sunder R. ; Olefsky, Jerrold ; Caro, Jose F. / Acute and chronic effect of insulin on leptin production in humans : Studies in vivo and in vitro. In: Diabetes. 1996 ; Vol. 45, No. 5. pp. 699-701.
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