Acute bacterial inflammation of the mouse prostate

Bayli J. Boehm, Sara A. Colopy, Travis Jerde, Christopher J. Loftus, Wade Bushman

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Background: Prostatic inflammation is gaining increasing attention as a potential etiologic factor in prostate cancer, benign prostatic hyperplasia, lower urinary tract symptoms, and CPPS. This study was performed to address the need for a well characterized model of acute prostatic inflammation that may be used to study the effect of acute inflammation on epithelial and stromal cell proliferation, voiding behavior, and neurovascular physiology. Methods: Uropathogenic E. coli 1677 was instilled transurethrally into adult C57BL/6J male mice. Prostates were analyzed at 1, 2, 3, 5, 7, or 14 days post-instillation and compared to saline-instilled and naïve controls. Time course and severity of inflammation were characterized by the quantity and type of inflammatory infiltrate present, hemorrhage, proliferation, and reactive hyperplasia. RT-PCR was performed to characterize inflammatory mediators including IL-1α, IL-1β, IL-1RA, IL-18, IL-6, IL-10, IL-8, TNFα, and COX-2. RESULTS Inflammation was evident in all lobes of the prostate with the DLP most severely affected. Infection consistently led to a significant increase in neutrophils and macrophages in the early stages of prostate infection, followed by lymphocytic inflammation at the later time points. Inflammation was accompanied by induction of several inflammatory genes, including IL-1 family members, IL-6, and COX-2, and induced a significant increase in epithelial proliferation and reactive hyperplasia in all three prostate lobes. Conclusions: Transurethral inoculation of uropathogenic E. coli 1677 reliably infects the mouse prostate, produces a significant inflammatory response, and induces quantifiable epithelial proliferation and reactive hyperplasia.

Original languageEnglish
Pages (from-to)307-317
Number of pages11
JournalProstate
Volume72
Issue number3
DOIs
StatePublished - Feb 2012

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Prostate
Inflammation
Interleukin-1
Uropathogenic Escherichia coli
Hyperplasia
Interleukin-6
Lower Urinary Tract Symptoms
Interleukin-18
Prostatic Hyperplasia
Stromal Cells
Infection
Interleukin-8
Interleukin-10
Prostatic Neoplasms
Neutrophils
Epithelial Cells
Macrophages
Cell Proliferation
Hemorrhage
Polymerase Chain Reaction

Keywords

  • E. coli
  • hyperplasia
  • inflammation
  • model

ASJC Scopus subject areas

  • Urology
  • Oncology

Cite this

Boehm, B. J., Colopy, S. A., Jerde, T., Loftus, C. J., & Bushman, W. (2012). Acute bacterial inflammation of the mouse prostate. Prostate, 72(3), 307-317. https://doi.org/10.1002/pros.21433

Acute bacterial inflammation of the mouse prostate. / Boehm, Bayli J.; Colopy, Sara A.; Jerde, Travis; Loftus, Christopher J.; Bushman, Wade.

In: Prostate, Vol. 72, No. 3, 02.2012, p. 307-317.

Research output: Contribution to journalArticle

Boehm, BJ, Colopy, SA, Jerde, T, Loftus, CJ & Bushman, W 2012, 'Acute bacterial inflammation of the mouse prostate', Prostate, vol. 72, no. 3, pp. 307-317. https://doi.org/10.1002/pros.21433
Boehm BJ, Colopy SA, Jerde T, Loftus CJ, Bushman W. Acute bacterial inflammation of the mouse prostate. Prostate. 2012 Feb;72(3):307-317. https://doi.org/10.1002/pros.21433
Boehm, Bayli J. ; Colopy, Sara A. ; Jerde, Travis ; Loftus, Christopher J. ; Bushman, Wade. / Acute bacterial inflammation of the mouse prostate. In: Prostate. 2012 ; Vol. 72, No. 3. pp. 307-317.
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