Acute effects of mitoxantrone on the template activity of isolated nuclei from the T-47D human breast tumor cell line

Nasser Chegini, Ahmad Safa, Michael T. Tseng

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The effect of mitoxantrone on the template activity of nuclei isolated from the T-47D human breast tumor cell line was investigated. The results suggest that mitoxantrone significantly inhibits total RNA synthesis of these nuclei in a concentration-dependent manner. At low drug concentrations (10-9 M and 10-7 M) RNA synthesis was inhibited by 21.9% and 41% compared to control values, respectively. Greater inhibition was observed when the mitoxantrone concentration was increased to 10-5 M or 10-4 M (56% and 77%, respectively). Experiments utilizing α-amanitin revealed that mitoxantrone inhibits RNA polymerase II activity in a concentration-dependent manner.

Original languageEnglish (US)
Pages (from-to)329-336
Number of pages8
JournalCancer Letters
Volume21
Issue number3
DOIs
StatePublished - 1984
Externally publishedYes

Fingerprint

Mitoxantrone
Tumor Cell Line
Breast Neoplasms
RNA
Amanitins
RNA Polymerase II
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Oncology

Cite this

Acute effects of mitoxantrone on the template activity of isolated nuclei from the T-47D human breast tumor cell line. / Chegini, Nasser; Safa, Ahmad; Tseng, Michael T.

In: Cancer Letters, Vol. 21, No. 3, 1984, p. 329-336.

Research output: Contribution to journalArticle

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