Acute effects of mitoxantrone on the template activity of isolated nuclei from the T-47D human breast tumor cell line

Nasser Chegini, Ahmad R. Safa, Michael T. Tseng

Research output: Contribution to journalArticle

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Abstract

The effect of mitoxantrone on the template activity of nuclei isolated from the T-47D human breast tumor cell line was investigated. The results suggest that mitoxantrone significantly inhibits total RNA synthesis of these nuclei in a concentration-dependent manner. At low drug concentrations (10-9 M and 10-7 M) RNA synthesis was inhibited by 21.9% and 41% compared to control values, respectively. Greater inhibition was observed when the mitoxantrone concentration was increased to 10-5 M or 10-4 M (56% and 77%, respectively). Experiments utilizing α-amanitin revealed that mitoxantrone inhibits RNA polymerase II activity in a concentration-dependent manner.

Original languageEnglish (US)
Pages (from-to)329-336
Number of pages8
JournalCancer Letters
Volume21
Issue number3
DOIs
StatePublished - Jan 1984

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ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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