Acute ethanol exposure suppresses the repair of O6-methylguanine DNA lesions in castrated adult male rats

D. M. Wilson, J. J. Tentler, J. P. Carney, T. M. Wilson, Mark Kelley

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Alcohol has clearly been associated with an increase of cancers in numerous tissues, including the respiratory tract, colon, rectum, liver, but especially the esophagus, larynx, pharynx, and mouth. Alcohol alone has not been shown to be a mutagen until it is converted to acetaldehyde and, therefore, alcohol presumably acts as a cocarcinogen. Previous data has shown that alcohol concentrations of 2% or greater inhibits DNA repair, and in light of the widespread consumption of alcoholic beverages with alcohol contents ranging from 4 to 5% (beer and wine coolers) to 50% (whiskey), interest in determining the mechanism(s) responsible for alcohol-induced carcinogenesis has heightened. Although previous studies, in intact rats, have investigated the effects of chronic alcohol exposure on some aspects of DNA repair, we have begun to address the effects of acute or 'binge' alcohol exposure on mammalian DNA repair. Toward this end, we report the inhibition of O6-methylguanine-DNA methyltransferase (MGMT) by a single intraperitoneal injection of 30% ethanol in adult male castrated rats. This inhibition lasted for at least 24 hr. We also observed a dose-response effect of ethanol on MGMT activity, again only in the castrated rats. The finding of ethanol's effect on MGMT activity in castrated and not intact rats implies a hormonal component of MGMT DNA repair response, which has only been alluded to in past research.

Original languageEnglish
Pages (from-to)1267-1271
Number of pages5
JournalAlcoholism: Clinical and Experimental Research
Volume18
Issue number5
DOIs
StatePublished - 1994

Fingerprint

Rats
Repair
Ethanol
Alcohols
DNA
Methyltransferases
DNA Repair
Beer
Alcoholic Beverages
Wine
Acetaldehyde
O-(6)-methylguanine
Mutagens
Larynx
Pharynx
Intraperitoneal Injections
Rectum
Respiratory System
Liver
Esophagus

Keywords

  • DNA Repair
  • Hormones
  • MGMT
  • O-Methylguanine DNA Methyltransferase

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Toxicology

Cite this

Acute ethanol exposure suppresses the repair of O6-methylguanine DNA lesions in castrated adult male rats. / Wilson, D. M.; Tentler, J. J.; Carney, J. P.; Wilson, T. M.; Kelley, Mark.

In: Alcoholism: Clinical and Experimental Research, Vol. 18, No. 5, 1994, p. 1267-1271.

Research output: Contribution to journalArticle

Wilson, D. M. ; Tentler, J. J. ; Carney, J. P. ; Wilson, T. M. ; Kelley, Mark. / Acute ethanol exposure suppresses the repair of O6-methylguanine DNA lesions in castrated adult male rats. In: Alcoholism: Clinical and Experimental Research. 1994 ; Vol. 18, No. 5. pp. 1267-1271.
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