Acute keratinocyte damage stimulates platelet-activating factor production

C. Alappatt, C. A. Johnson, K. L. Clay, Jeffrey Travers

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Recent evidence suggests that the phosphocholine-derived lipid mediator platelet-activating factor (PAF) is involved in keratinocyte function and cutaneous inflammation. PAF is found in various inflammatory skin diseases, and intradermal injection of PAF directly results in cutaneous inflammation. Keratinocytes also synthesize PAF and related 1-acyl species in response to ionophores, cytokines and growth factors, and in response to activation of the epidermal PAF receptor. Since keratinocytes are routinely exposed to potential damage by thermal or oxidative stressors with resultant induction of cutaneous inflammation, the objective of these studies was to assess whether exogenous thermal or oxidative damage can induce the production of PAF and related 1-acyl species. Cells of the immortalized human keratinocyte cell line HaCaT were subjected to acute heat or cold, or treatment with the pro-oxidant lipid tertiary butyl hydroperoxide, and PAF and 1-palmitoyl-2-acetyl-GPC were measured by gas chromatography/mass spectrometry. We report that these diverse toxic stimuli resulted in the accumulation of these biologically active lipids. These studies suggest that the PAF system is involved in the inflammatory response seen following acute epidermal damage.

Original languageEnglish
Pages (from-to)256-259
Number of pages4
JournalArchives of Dermatological Research
Volume292
Issue number5
StatePublished - 2000

Fingerprint

Platelet Activating Factor
Keratinocytes
Hot Temperature
Inflammation
Lipids
Skin
Intradermal Injections
Phosphorylcholine
Poisons
Ionophores
Skin Diseases
Gas Chromatography-Mass Spectrometry
Hydrogen Peroxide
Reactive Oxygen Species
Intercellular Signaling Peptides and Proteins
Cytokines
Cell Line

Keywords

  • Keratinocytes
  • Mass spectrometry
  • Oxidative stress
  • Platelet-activating factor

ASJC Scopus subject areas

  • Dermatology

Cite this

Acute keratinocyte damage stimulates platelet-activating factor production. / Alappatt, C.; Johnson, C. A.; Clay, K. L.; Travers, Jeffrey.

In: Archives of Dermatological Research, Vol. 292, No. 5, 2000, p. 256-259.

Research output: Contribution to journalArticle

Alappatt, C. ; Johnson, C. A. ; Clay, K. L. ; Travers, Jeffrey. / Acute keratinocyte damage stimulates platelet-activating factor production. In: Archives of Dermatological Research. 2000 ; Vol. 292, No. 5. pp. 256-259.
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