Addition of carbohydrate side chains at novel sites on influenza virus hemagglutinin can modulate the folding, transport, and activity of the molecule

Patricia Gallagher, J. Henneberry, I. Wilson, J. Sambrook, M. J. Gething

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

We have constructed and expressed a series of mutant influenza virus hemagglutinins, each containing a new consensus site for glycosylation in addition to the seven sites found on the wild-type protein. Oligosaccharide side chains were added with high efficiency at four of the five novel sites, located on areas of the protein's surface that are not normally shielded by carbohydrate. Investigations of the structure, intracellular transport, and biological activities of the mutant hemagglutinin molecules indicated that (a) supernumerary carbohydrate side chains can be used to shield or disrupt functional epitopes on the surface of hemagglutinin, and (b) the presence of an additional oligosaccharide may cause temperature-dependent defects in the transport of the glycoprotein. We discuss the addition of supernumerary oligosaccharides as a general tool for shielding chosen areas of the surface of proteins that enter or traverse the secretory pathway.

Original languageEnglish (US)
Pages (from-to)2059-2073
Number of pages15
JournalJournal of Cell Biology
Volume107
Issue number6 I
StatePublished - 1988
Externally publishedYes

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Hemagglutinins
Orthomyxoviridae
Oligosaccharides
Carbohydrates
Membrane Proteins
Biological Transport
Secretory Pathway
Glycosylation
Epitopes
Glycoproteins
Temperature
Proteins

ASJC Scopus subject areas

  • Cell Biology

Cite this

Addition of carbohydrate side chains at novel sites on influenza virus hemagglutinin can modulate the folding, transport, and activity of the molecule. / Gallagher, Patricia; Henneberry, J.; Wilson, I.; Sambrook, J.; Gething, M. J.

In: Journal of Cell Biology, Vol. 107, No. 6 I, 1988, p. 2059-2073.

Research output: Contribution to journalArticle

Gallagher, Patricia ; Henneberry, J. ; Wilson, I. ; Sambrook, J. ; Gething, M. J. / Addition of carbohydrate side chains at novel sites on influenza virus hemagglutinin can modulate the folding, transport, and activity of the molecule. In: Journal of Cell Biology. 1988 ; Vol. 107, No. 6 I. pp. 2059-2073.
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