Adeno-associated virus 2-mediated gene transfer in vivo: Organ-tropism and expression of transduced sequences in mice

Selvarangan Ponnazhagan, Pinku Mukherjee, Mervin Yoder, Xu Shan Wang, Shang Zhen Zhou, Johanne Kaplan, Samuel Wadsworth, Arun Srivastava

Research output: Contribution to journalArticle

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Abstract

Adeno-associated virus 2 (AAV), a non-pathogenic human parvovirus, is gaining attention as a vector for its potential use in human gene therapy. However, few studies have examined the safety and the efficacy of this vector system in vivo. We report here that recombinant AAV vectors, when directly injected intravenously in mice, accumulated predominantly in liver cells, suggesting that AAV may possess in vivo organ-tropism for liver. The transduced lacZ reporter gene was expressed in hepatocytes in the liver and, at the level examined, did not appear to induce any detectable cytotoxic T lymphocyte response against βGal. AAV-mediated transduction of murine hematopoietic progenitor cells ex vivo followed by transplantation into lethally irradiated syngeneic mice also revealed high-efficiency gene transfer into progeny cells without any observable cytotoxicity or deleterious effect. The transduced reporter gene sequences were also expressed in mice in vivo. The AAV-based vectors may thus prove useful as a potentially safe alternative to the more commonly used retrovirus- and adenovirus-based vector systems.

Original languageEnglish
Pages (from-to)203-210
Number of pages8
JournalGene
Volume190
Issue number1
DOIs
StatePublished - Apr 29 1997

Fingerprint

Dependovirus
Tropism
Genes
Reporter Genes
Liver
Parvovirus
Lac Operon
Cytotoxic T-Lymphocytes
Retroviridae
Hematopoietic Stem Cells
Adenoviridae
Genetic Therapy
Hepatocytes
Transplantation
Safety

Keywords

  • Bone marrow transplantation
  • Gene delivery
  • Gene therapy
  • Hematopoiesis
  • Recombinant DNA
  • Viral vectors

ASJC Scopus subject areas

  • Genetics

Cite this

Ponnazhagan, S., Mukherjee, P., Yoder, M., Wang, X. S., Zhou, S. Z., Kaplan, J., ... Srivastava, A. (1997). Adeno-associated virus 2-mediated gene transfer in vivo: Organ-tropism and expression of transduced sequences in mice. Gene, 190(1), 203-210. https://doi.org/10.1016/S0378-1119(96)00576-8

Adeno-associated virus 2-mediated gene transfer in vivo : Organ-tropism and expression of transduced sequences in mice. / Ponnazhagan, Selvarangan; Mukherjee, Pinku; Yoder, Mervin; Wang, Xu Shan; Zhou, Shang Zhen; Kaplan, Johanne; Wadsworth, Samuel; Srivastava, Arun.

In: Gene, Vol. 190, No. 1, 29.04.1997, p. 203-210.

Research output: Contribution to journalArticle

Ponnazhagan, S, Mukherjee, P, Yoder, M, Wang, XS, Zhou, SZ, Kaplan, J, Wadsworth, S & Srivastava, A 1997, 'Adeno-associated virus 2-mediated gene transfer in vivo: Organ-tropism and expression of transduced sequences in mice', Gene, vol. 190, no. 1, pp. 203-210. https://doi.org/10.1016/S0378-1119(96)00576-8
Ponnazhagan, Selvarangan ; Mukherjee, Pinku ; Yoder, Mervin ; Wang, Xu Shan ; Zhou, Shang Zhen ; Kaplan, Johanne ; Wadsworth, Samuel ; Srivastava, Arun. / Adeno-associated virus 2-mediated gene transfer in vivo : Organ-tropism and expression of transduced sequences in mice. In: Gene. 1997 ; Vol. 190, No. 1. pp. 203-210.
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