Adenovirus‐mediated gene transfer of wild‐type p53 results in melanoma cell apoptosis in vitro and in vivo

Corrado Cirielli, Teresa Riccioni, Chunlin Yang, Roberto Pili, Torsten Gloe, Joan Chang, Kyosuke Inyaku, Antonino Passaniti, Maurizio C. Capogrossi

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Gene transfer techniques may provide efficient treatment for a variety of malignant neoplasms. A replication‐deficient adeno‐virus (Ad) vector which carries the cDNA for wild‐type p53 (AdCMV.p53) was tested for its in vitro and in vivo effects on the growth of murine melanoma cell line B16‐G3.26 and human melanoma cell line SK‐MEL‐24. The growth of B16‐G3.26 cells infected with AdCMV.p53 was inhibited when compared to the uninfected cells or cells infected with the control vector AdCMV.NLSβgal. Similarly, the growth of SK‐MEL‐24 cells infected with AdCMV.p53 was also below that of AdCMV.NLSβgal‐infected and uninfected controls. DNA laddering using agarose gel electrophoresis and in situ labeling of DNA fragmentation (TUNEL) showed that AdCMV.p53‐infected murine and human melanoma cells underwent apoptosis. Nude mice injected s.c. either with B16‐G3.26 cells or with SK‐MEL‐24 cells developed localized tumors. These tumors were subsequently infiltrated with either AdCMV.p53, AdCMV.NLSβgal or saline alone. One week after infection, B16‐G3.26 tumors exposed to AdCMV.p53 were 2.5 times smaller than control tumors and exhibited DNA fragmentation. A similar growth‐inhibitory effect of AdCMV.p53 was observed with SK‐MEL‐24 tumors. Thus, Ad‐mediated wild‐type p53 overexpression resulted in melanoma cell apoptosis and inhibition of melanoma growth in vitro and in vivo. These gene therapy approaches may be useful in targeting rapidly growing, malignant melanomas in a clinical setting. © 1995 Wiley‐Liss, Inc.

Original languageEnglish (US)
Pages (from-to)673-679
Number of pages7
JournalInternational Journal of Cancer
Volume63
Issue number5
DOIs
StatePublished - 1995
Externally publishedYes

Fingerprint

Melanoma
Apoptosis
Genes
Neoplasms
DNA Fragmentation
Growth
Cell Line
Gene Transfer Techniques
In Vitro Techniques
Agar Gel Electrophoresis
In Situ Nick-End Labeling
Adenoviridae
Nude Mice
Genetic Therapy
Complementary DNA
DNA
Infection

ASJC Scopus subject areas

  • Medicine(all)
  • Oncology
  • Cancer Research

Cite this

Adenovirus‐mediated gene transfer of wild‐type p53 results in melanoma cell apoptosis in vitro and in vivo. / Cirielli, Corrado; Riccioni, Teresa; Yang, Chunlin; Pili, Roberto; Gloe, Torsten; Chang, Joan; Inyaku, Kyosuke; Passaniti, Antonino; Capogrossi, Maurizio C.

In: International Journal of Cancer, Vol. 63, No. 5, 1995, p. 673-679.

Research output: Contribution to journalArticle

Cirielli, C, Riccioni, T, Yang, C, Pili, R, Gloe, T, Chang, J, Inyaku, K, Passaniti, A & Capogrossi, MC 1995, 'Adenovirus‐mediated gene transfer of wild‐type p53 results in melanoma cell apoptosis in vitro and in vivo', International Journal of Cancer, vol. 63, no. 5, pp. 673-679. https://doi.org/10.1002/ijc.2910630512
Cirielli, Corrado ; Riccioni, Teresa ; Yang, Chunlin ; Pili, Roberto ; Gloe, Torsten ; Chang, Joan ; Inyaku, Kyosuke ; Passaniti, Antonino ; Capogrossi, Maurizio C. / Adenovirus‐mediated gene transfer of wild‐type p53 results in melanoma cell apoptosis in vitro and in vivo. In: International Journal of Cancer. 1995 ; Vol. 63, No. 5. pp. 673-679.
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AU - Cirielli, Corrado

AU - Riccioni, Teresa

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AU - Pili, Roberto

AU - Gloe, Torsten

AU - Chang, Joan

AU - Inyaku, Kyosuke

AU - Passaniti, Antonino

AU - Capogrossi, Maurizio C.

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AB - Gene transfer techniques may provide efficient treatment for a variety of malignant neoplasms. A replication‐deficient adeno‐virus (Ad) vector which carries the cDNA for wild‐type p53 (AdCMV.p53) was tested for its in vitro and in vivo effects on the growth of murine melanoma cell line B16‐G3.26 and human melanoma cell line SK‐MEL‐24. The growth of B16‐G3.26 cells infected with AdCMV.p53 was inhibited when compared to the uninfected cells or cells infected with the control vector AdCMV.NLSβgal. Similarly, the growth of SK‐MEL‐24 cells infected with AdCMV.p53 was also below that of AdCMV.NLSβgal‐infected and uninfected controls. DNA laddering using agarose gel electrophoresis and in situ labeling of DNA fragmentation (TUNEL) showed that AdCMV.p53‐infected murine and human melanoma cells underwent apoptosis. Nude mice injected s.c. either with B16‐G3.26 cells or with SK‐MEL‐24 cells developed localized tumors. These tumors were subsequently infiltrated with either AdCMV.p53, AdCMV.NLSβgal or saline alone. One week after infection, B16‐G3.26 tumors exposed to AdCMV.p53 were 2.5 times smaller than control tumors and exhibited DNA fragmentation. A similar growth‐inhibitory effect of AdCMV.p53 was observed with SK‐MEL‐24 tumors. Thus, Ad‐mediated wild‐type p53 overexpression resulted in melanoma cell apoptosis and inhibition of melanoma growth in vitro and in vivo. These gene therapy approaches may be useful in targeting rapidly growing, malignant melanomas in a clinical setting. © 1995 Wiley‐Liss, Inc.

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